健康和感染黄龙病沙糖桔嫩梢挥发性成分的分析  

Analysis of volatile compounds from young shoots of non- infected and huanglongbing- infected citrus

作　　者：杨玉枝[1] 徐迪[1] 岑伊静[1] 

机构地区：[1]华南农业大学昆虫生态研究室,广州510642

出　　处：《环境昆虫学报》2015年第2期328-333,共6页Journal of Environmental Entomology

基　　金：广东省科技计划(2012A020200016);公益性行业(农业)科研专项(201003067-01)

摘　　要：亚洲柑桔木虱Diaphorina citri Kuwayama为黄龙病的主要媒介昆虫。与健康柑桔相比,带有嫩梢并感染黄龙病的柑桔对该木虱的吸引作用较强。本文采用顶空固相微萃取和气质联用(GC/MS)分析健康和黄龙病沙糖桔嫩梢挥发物成分。结果表明,健康沙糖桔和黄龙病沙糖桔挥发性主要成分都是烯烃类、醇类,其中健康柑桔嫩梢挥发物的主要组分为β-榄香烯(17.0%)、β-罗勒烯(15.22%)、β-水芹烯(14.29%),而黄龙病嫩梢挥发物的主要组分为β-水芹烯(59.98%)、β-榄香烯(9.04%)。健康和黄龙病沙糖桔释放的挥发性化学物质种类差异较大,同种化合物的含量差异也较大。Diaphorina citri Kuwayama is a primary vector of citrus huanglongbing（ HLB）. It has been proved that the HLB- infected citrus with young shoots were more attracted to D. citri than non- infected citrus. In this study,the volatile compounds were extracted from young shoots of HLB- infected and non- infected citrus respectively using headspace solid phase micro- extraction method. The extracted compounds were then analyzed by gas chromatography / mass spectrometry（ GC / MS） combined technology. The result showed that the primary volatile compounds from both HLB- infected and non-infected citrus were olefins and alcohols. The main components of the compounds from non- infected shoots were β- Elemene,β- Ocimene and β- Phellandrene,whose relative content were 17. 0%,15. 22% and 14. 29% respectively. The main components of compounds from non- infected shoots wereβ- Phellandrene and β- Elemene,whose relative content were 59. 98% and 9. 04% respectively. The result demonstrated that chemical components of the volatile compounds were greatly different between non- infected and HLB- infected citrus,and the contents of the same component were also different.

关 键 词：黄龙病 寄主挥发物 GC-MS 亚洲柑桔木虱 

分 类 号：Q965[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]