锰超氧化物歧化酶9 Ala／Val 基因多态性对冠心病的影响  

Effects of Manganese Superoxide Dismutase 9 Ala/Val Genetic Polymorphisms on Coronary Heart Disease

作　　者：姚创利[1] 赵佳[1] 黎阳[1] 黎波[1] 陆华[1] 白妮[1] 刘琳[1] 辛渭川[2] 姜小建[1] 

机构地区：[1]西安市中心医院检验科,西安710003 [2]西安市中心医院心内科,西安710003

出　　处：《现代检验医学杂志》2015年第2期1-2,6,共3页Journal of Modern Laboratory Medicine

基　　金：陕西省科学技术研究发展计划项目(项目编号2013K12-02-10)。

摘　　要：目的：研究锰超氧化物歧化酶（Mn-SOD）9 Ala／Val 基因多态性与 T-SOD 和 Mn-SOD 活性的关系以及对冠心病（CHD）的影响。方法共82例冠心病患者和57例正常人参与研究，应用测序法检测两组的 Mn-SOD 9 Ala／Val 基因多态性的基因型，应用比色法检测两组的血清 T-SOD 和 Mn-SOD 活性。结果冠心病组的血清 T-SOD 和 Mn-SOD 活性低于对照组（t＝4.83，6.57，P 均＜0.05），冠心病组的 Mn-SOD 9 VV 基因型和 V 等位基因频率高于对照组（χ^2＝4.75，P ＜0.05），Mn-SOD 9 VV 基因型的血清 T-SOD 和 Mn-SOD 活性低于 Mn-SOD 9 AA 基因型（t＝2.96，3.11，P 均＜0.05）。结论冠心病患者的血清 T-SOD 和 Mn-SOD 活性降低，Mn-SOD 9 Ala／Val 基因多态性通过影响 Mn-SOD 的活性进而参与冠心病的发病。Objective To study associations between manganese superoxide dismutase 9 Ala/Val （Mn-SOD 9 Ala/Val）genetic polymorphism and total superoxide dismutase （T-SOD）and Mn-SOD activity and the impact on coronary heart disease （CHD）were studied.Methods There were 82 CHD patients and 57 controls in this research.Sequencer was used to identify the genotype of Mn-SOD 9 Ala/Val genetic polymorphism and colorimeter was used to detect the serum T-SOD and Mn-SOD activity.Results Compared with the control group,the serum T-SOD and Mn-SOD activity of the CHD group was significantly reduced（t=4.83,6.57,P all〈0.05）,while the VV genotype and V allele of Mn-SOD 9 Ala/Val genetic poly-morphism of the CHD group were higher （χ^2 =4.75,P 〈0.05）.The serum T-SOD and Mn-SOD activity of the Mn-SOD 9 VV genotype was significantly lower than the Mn-SOD 9 AA genotype（t=2.96,3.11,P all〈0.05）.Conclusion The ser-um T-SOD and Mn-SOD activity in the CHD patients was reduced.Mn-SOD 9 Ala/Val genetic polymorphism was involved in the pathogenesis of CHD by influencing the Mn-SOD activity.

关 键 词：锰超氧化物歧化酶 冠心病 基因多态性 

分 类 号：R541.4[医药卫生—心血管疾病] Q781[医药卫生—内科学]