PCR-SBT 与 IMS-ELISA 法在强直性脊柱炎患者 HLA-B27检测中的比较  

Comparison Analysis of HLA-B27 Detected by PCR-SBT and IMS-ELISA in AS Patients

作　　者：孙灵迪[1,2] 梅传忠[1] 王平均[2] 武晓茜[2] 邵先安[1,2] 

机构地区：[1]蚌埠医学院,安徽蚌埠233030 [2]解放军第 123 医院检验病理科,安徽蚌埠233015

出　　处：《现代检验医学杂志》2015年第2期16-18,共3页Journal of Modern Laboratory Medicine

基　　金：南京军区医学科技课题资助项目(12MB015)。

摘　　要：目的：比较聚合酶链反应-直接测序法（polymerase chain reaction sequence-based typing，PCR-SBT）和磁珠酶联免疫法（immunomagnetic separation and enzyme-linked immunosorbent assay，IMS-ELISA）检测人类白细胞抗原 B27（human leukocyte antigen B27，HLA-B27）在强直性脊柱炎（ankylosing spondylitis，AS）临床诊断中的价值。方法应用 PCR-SBT法和 IMS-ELISA 法对120例疑似 AS 患者外周血进行 HLA-B27检测。以 SPSS17.0软件的配对χ^2检验和受试者工作特征曲线下面积对两种方法检测 HLA-B27在 AS 诊断中的价值进行评价。结果120例疑似 AS 患者中，PCR-SBT 和 IMS-ELISA 法 HLA-B27检测阳性率分别为45.83％（55／120）和37.50％（45／120）。两种方法检测 HLA-B27差异有统计学意义（χ2＝59.455，P ＝0.000）。PCR-SBT 法敏感度和特异度分别为96.36％和96.92％；IMS-ELISA 法的敏感度和特异度分别为69.09％和89.23％。两者的 AUC 分别为0.966和0.792。结论与 IMS-ELISA 法相比，在 AS 的临床诊断中PCR-SBT 法检测 HLA-B27的敏感度和特异度更高，方法更优。Objective To compare PCR-SBT to IMS-ELISA in the HLA-B27 detection in the ankylosing spondylitis （AS）patients.Methods Simultaneously,PCR-SBT and IMS-ELISA were used to detect the HLA-B27 expression in peripheral blood samples which were suspected patients with AS from 120 cases.Chisquare test of paired design and the area under curve of receiver operating characteristics of SPSS17.0 software were used to evaluate the value of PCR-SBT and IMS-ELISA in HLA-B27 detection of AS patients.Results Among 120 cases of suspected patients with AS,the positive rates of HLA-B27 detected by PCR-SBT and IMS-ELISA were 45.83%（55/120）and 37.50% （45/120）,respectively.There was statistical difference between the two methods in the HLA-B27 detection （χ^2 =59.455,P =0.000）.The sensibility and spe-cificity of PCR-SBT were 96.36% and 96.92%,respectively.While the sensibility and the specificity of IMS-ELISA were 69.09% and 89.23%,respectively.Area under the curve of two methods were 0.966 and 0.792,respectively.Conclusion In comparison with IMS-ELISA,the sensibility and the specificity of PCR-SBT in HLA-B27 detection were higher in AS diag-nosis,that is to say,PCR-SBT is better in HLA-B27 detection and AS diagnosis.

关 键 词：聚合酶链反应-直接测序法 磁珠酶联免疫法 人类白细胞抗原B27 强直性脊柱炎 

分 类 号：R593.23[医药卫生—内科学] Q503[医药卫生—临床医学]