阴道分泌物对女性尿液干化学白细胞酯酶测定的影响  

Influence of Vaginal Discharge on Dry Chemistry Determination of Leukocyte Esterase in Female Urine

作　　者：吴跃军[1] 季明德[1] 刘淑杰[2] 

机构地区：[1]江苏省中医院检验科,南京210029 [2]南京市妇幼保健院,南京210004

出　　处：《现代检验医学杂志》2015年第2期163-164,共2页Journal of Modern Laboratory Medicine

摘　　要：目的探讨阴道分泌物对女性尿液干化学白细胞酯酶测定的影响。方法收集正常阴道分泌物Ⅰ，Ⅱ度标本21份和30份，并测定其中的白细胞酯酶和鳞状上皮细胞；再收集正常阴道分泌物标本，从中分离出鳞状上皮细胞，并配制成20个／μl和60个／μl两个浓度的鳞状上皮细胞混悬液。将其加入正常女性尿液后进行干化学白细胞酯酶泱3定；对于干化学白细胞酯酶测定强阳性而尿沉渣白细胞计数正常的患者做外阴清洗后再取中段尿做尿常规检验。结果正常阴道分泌物中有66．7％的白细胞酯酶结果呈阳性，且均有＋＋-＋＋＋＋的鳞状上皮细胞（镜检）；正常尿液组和加入低浓度（20个／μl）及高浓度（60个／μl）鳞状上皮细胞尿液组的尿液干化学白细胞酯酶测定差异无统计学意义（P〉0．05）；再次中段尿的鳞状上皮细胞和白细胞干化学阳性率及强度较第一次尿明显降低，差异有统计学意义（P〈0．05），而白细胞计数无明显区别，差异无统计学意义（P〉0．05）。结论阴道鳞状上皮细胞不会对尿液干化学白细胞酯酶的测定产生影响。但女性自身阴道分泌物中的白细胞酯酶对其尿液中的白细胞酯酶的测定有很大影响。Objective The present study is to investigate the influence of vaginal discharge on dry chemistry determination of leukocyte esterase in female urine. Methods Collected 20 and 30 normal vaginal discharge samples. Which humoral routine test degree were Ⅰ and Ⅱ correspondingly,and then analyze leukocyte esterase and squamous epithelial cells in these normal vaginal discharge samples. Collected normal vaginal discharge samples again and isolate the vaginal squamous epithelial cells from those normal vaginal discharge samples. Made two concentration of squamous epithelial cell suspension liquid （20μl and 60μl） and added these liquid. To normal female urine and analyze the drying chemical leukocyte esterase in those urine. Cleaned the vulva of those patients with normal leukocyte counts in urine sediment determination, whose dry chemical determination of leukocyte esterase was strong positive, perform the routine urine test with her middle urine. Results The results of the determination of leukocyte esterase in normal vaginal discharge samples were 66.7 % positive. And there were ＋＋＋ squamous epithelial cells in all samples （microscopy）. No significant difference was observed in the examination of dry chemistry leukocyte esterase among the normal female urine group,low （20/μl） and high （60/μ1） concentrations of squamous epithelial cell urine group （P〉0.05）. The counts of squamous epithelial cells and the rate of positive and intensity of dry chemical leukocyte esterase in the middle of second urine were significantly lower than before,and the differences are statistically significant （P〈0.05）. But there was no significant difference for leukocytes counts. Conclusion Urine squamous epithelial cells had no influence on the detection of leukocyte esterase by dry chemistry. However,the leukocyte esterase in the vaginal discharge greatly influenced the examination of the leukocyte esterase in urine.

关 键 词：白细胞酯酶 干化学 鳞状上皮细胞 阴道分泌物 

分 类 号：R446.19[医药卫生—诊断学]