荆芥穗提取物体外抗呼吸道合胞病毒有效部位研究  

Study on Antiviral Components in vitro of Schizonepeta tenuifolia Briq.

作　　者：张霞[1] 周长征[2] 姚梅悦[1] 陈飞[1] 

机构地区：[1]山东中医药大学 [2]山东中医药大学药学院,山东济南250355

出　　处：《山东中医杂志》2015年第3期213-215,共3页Shandong Journal of Traditional Chinese Medicine

摘　　要：目的 :研究荆芥穗提取物不同部位对呼吸道合胞病毒(RSV)的体外抗病毒活性。方法:荆芥穗提取液经过D101大孔树脂吸附后,依次用水、25%乙醇、50%乙醇、75%乙醇进行洗脱。采用MA-104细胞感染模型,以利巴韦林为阳性对照药,初步检测其体外抗病毒活性。在显微镜下观察细胞病变,酶标仪测中性红染色OD值,计算治疗指数(TI)。结果:水洗脱液的1,2,3号均有抗RSV的效果,其中尤以2号最为有效,其TI为63.55。结论:荆芥穗的水溶性成分对RSV病毒有明显的抑制作用。Objective：To study antiviral activities of different components extracted from Sehizonepetae tenuifolia Briq. on respiratory syncytial virus（RSV） in vitro. Methods：The extraction solution of Schiznepeta tenuifolia Briq. was absorbed by D101 macroporous resin,and then washing it with water,25% ethanol,50% ethanol and 75% ethanol respectively. Through MA-104 cells infection model and using Ribavirin as a positive control,anti-RSV activity was detected in vitro, Cytopathic effect was carried out by microscope,neutral red staining OD value was measured by ELIASA and therapeutic index（TI） was calculated. Results：The washing liquid of No. 1,2,3 had the effects of anti-RSV. The most effective liquid was No.2,its TI was 63.55. Conclusion：Water-soluble components of Schizonepeta tenuifolia Briq. has obvious effects of anti-RSV.

关 键 词：荆芥穗 呼吸道合胞病毒 体外抗病毒 有效部位 

分 类 号：R284.2[医药卫生—中药学]