云南汉族人群CTLA-4基因多态性与自身免疫性甲状腺疾病的关系  

Relationship between T-lymphocyte-associated antigen-4 Gene Polymorphism in Yunnan Han Population and Autoimmune Thyroid Disease

作　　者：王毅鹏[1] 彭葆坤[1] 朱丽芹[1] 翁晓春[1] 王瑛[1] 唐哲[1] 

机构地区：[1]昆明医科大学附属延安医院,云南昆明650051

出　　处：《中国医学创新》2015年第12期14-17,共4页Medical Innovation of China

基　　金：国家自然科学基金(地区科学基金项目)(81460154)

摘　　要：目的:探讨云南地区汉族人群自身免疫性甲状腺疾病(AITD)与细胞毒性T淋巴细胞相关抗原4(CTLA-4)第1外显子第17密码子49位点A/G基因多态性的相关性。方法:应用多聚酶链反应—限制性片断长度多态性分析(PCR-RFLP)的实验方法,检测82例Graves病(GD)患者,51例桥本氏甲状腺炎(HT)患者和80例健康人外周血CTLA-4基因第1外显子的第17密码子49位点的基因型并行分析。结果:GD组GG基因型的频率及等位基因G的频率明显高于健康对照组(X2=9.353,P=0.009;X2=7.548,P=0.006);而HT组GG基因型及G等位基因的分布频率与健康对照组比较差异无统计学意义(X2=0.062,P=0.969;X2=0.046,P=0.830)。结论:CTLA-4基因第1外显子的第17密码子49位点可能是云南汉族GD的易感候选基因。Objective：To investigate the correlation of gene polymorphism Cytotoxic T lymphocyteassociated Antigen4 （CTLA-4）at position 49 in exon 1 codon 17 with autoimmune thyroid disease （AITD）.Method：The gene polymorphism CTLA-4 at position 49 in exon 1 codon 17 were conducted using polymerase chain reaction with sequence specific primers in the specimen of peripheral blood.82 patients with Graves disease （GD） and 51 patients with Hashimoto’s thyroiditis （HT） were recruited.80 healthy volunteers constituted the control group.Result：The gene frequencies of CTLA-4 at position 49 in exon 1 codon 17 GG genotype and G alleles were significantly higher in GD groupthan in control group（ χ^2=9.353,P=0.009; χ^2=7.548,P=0.006）.The gene frequencies of CTLA-4 at position 49 in exon 1 codon 17 GG genotype and G alleles were not significantly different in HT group and control group（ χ^2=0.062, P=0.969; χ^2=0.046,P=0.830）.Conclusion：The gene polymorphism CTLA-4 at position 49 in exon 1 codon 17 may be an independent risk factor for AITD.

关 键 词：自身免疫性甲状腺疾病 细胞毒性T淋巴细胞相关抗原4 基因多态性 

分 类 号：R581[医药卫生—内分泌]