补阳还五汤对脑缺血大鼠血清TNF-ɑ、IL-6、CRP的影响研究  

Influence of Buyanghuanwu Decoction on the Expression of TNF-ɑ,IL-6,CRP in Cerebral Ischemia Rats

作　　者：吴旋[1] 汪欣[1] 陈云欢[1] 蔡梅玉[1] 程颖[1] 

机构地区：[1]福建中医药大学附属康复医院,福建福州350003

出　　处：《中国医学创新》2015年第12期30-32,共3页Medical Innovation of China

基　　金：福建中医药大学重点学科(脑血管疾病康复的基础和临床研究)专项校管课题(X2014076-学科);福建省中青年教师教育科研项目(科技B类)(JB14056);福建省卫生计生委青年科研课题(2014-2-37)

摘　　要：目的:探讨补阳还五汤对MCAO(大脑中动脉线栓法)大鼠血清TNF-ɑ、IL-6、CRP的影响。方法:60只大鼠随机分为四组:假手术组、模型组、补阳还五汤低剂量组、补阳还五汤高剂量组。Garcia JH评分标准评价受试大鼠的神经功能,化学发光法检测大鼠外周血清TNF-a、IL-6的水平,免疫比浊法检测大鼠血清CRP水平。结果:术后第7天,补阳还五汤低剂量组和高剂量组大鼠神经功能得到一定程度的恢复,与模型组比较差异有统计学意义(P<0.05),同时低剂量组和高剂量组比较差异也有统计学意义(P<0.05)。补阳还五汤低剂量组、高剂量组的TNF-a与模型组比较差异有统计学意义(P<0.05);低剂量组的IL-6、CRP与模型组比较差异均无统计学意义(P>0.05);高剂量组的IL-6、CRP与模型组比较差异均有统计学意义(P<0.05)。结论:补阳还五汤可下调MCAO大鼠TNF-a、IL-6、CRP水平,通过减轻炎症作用,达到神经保护的作用。Objective：To discuss the influence of Buyanghuanwu decoction on the expression of TNF-ɑ, IL-6,CRP in MCAO rats.Method：60 rats were divided into four groups：control group, model group,low-dose of Buyanghuanwu decoction group and high-dose of Buyanghuanwu decoction group.Motor nerve function of rat was evaluated by using Garcia JH score ,serum TNF-ɑ,IL-6 were detected by using bioluminescence and serum CRP were detected by using immunoturbidimetry.Result：7 days after surgery,compared with model group, nerve function of rats recovered in low-dose group and high-dose group,with significant difference（P〈0.05）.There was significant difference betweenlow-dose group and high-dose group（P〈0.05）.Compared with model group,there was significant difference of TNF-ɑ in low-dose group and high-dose group（P〈0.05）.Compared with model group,there was no difference of IL-6,CRP in low-dose group（P〉0.05） ,with significant difference in high-dose group（P〈0.05） .Conclusion：In order to protect nerve function,Buyanghuanwu decoction can reduce TNF-ɑ,IL-6,CRP and relieve inflammation.

关 键 词：补阳还五汤 神经功能 炎症因子 

分 类 号：R285.5[医药卫生—中药学]