一株高产PHB蜡状芽孢杆菌的分离筛选与鉴定  

Isolation and Identification of a Poly-β-Hydroxybutyrate Yielding Bacillus Cereus Strain

作　　者：邓加聪[1] 徐慧诠[1] 詹兴代 苏泉培 

机构地区：[1]福建师范大学福清分校海洋与生化工程学院,福建福清350300

出　　处：《福建师大福清分校学报》2015年第2期91-96,共6页Journal of Fuqing Branch of Fujian Normal University

基　　金：福建师范大学福清分校校级重点学科建设基金(编号:20110346);福建省大学生创新项目(编号:SCX-2013026)

摘　　要：通过尼罗蓝平板筛选、苏丹黑染色法及摇瓶复筛,从土壤中分离筛选到一株高产PHB的菌株FQB6,经形态观察、生理生化实验及16S r DNA遗传分析,鉴定该菌株为蜡状芽孢杆菌(Bacillus cereus)。进一步研究菌株的生长规律,发现菌株的延迟期为0-36h,对数生长期为36h-60h,稳定期为60h-96h,当培养时间为84h时,PHB产量和生物量均达到最大,分别为1.926g/L和1.05g/L。The strain FQB6 producing PHB isolated from soil by Nile blue plate, Sultan black staining and shake flask screening was identified as Bacillus cereus according to its morphological, physiological properties and 16S rDNA gene analysis. Growth regularity of further research on strain, strain found the lag phase, logarithmic growth and stationary phase was 0 -36h, 36h -60h and 60h -96h respectively when the incubation time for 84h, the yield of PHB and weight of dry cell reached the maximum, 1. 926g/L and 1.05g/L respectively.

关 键 词：聚Β-羟基丁酸 分离 筛选 蜡状芽孢杆菌 

分 类 号：Q93[生物学—微生物学]