鼠疫耶尔森菌和假结核耶尔森菌基因鉴别方法的建立及评价  

Establishment and evaluation of identification method for Yersinia pestis and Yersinia pseudotuberculosis

作　　者：石国祥[1] 张政[1] 梅玲玲[1] 陈劲华[2] 梅盛华[3] 金大智[1] 张志凯[4] 王宇萌 张孝和[5] 罗芸[1] 孙继民[1] 俞东征[4] 夏连续[4] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051 [2]浙江省义乌市疾病预防控制中心 [3]浙江省龙泉市疾病预防控制中心 [4]中国疾病预防控制中心传染病预防控制所 [5]浙江省温州市疾病预防控制中心

出　　处：《中华流行病学杂志》2015年第5期496-500,共5页Chinese Journal of Epidemiology

摘　　要：目的建立鼠疫耶尔森菌和假结核耶尔森菌基因鉴别方法。方法依据鼠疫菌、假结核菌特有的基因组序列[“疫岛（PeI）”和“假岛（Psi）”]，与已公布的12株鼠疫菌和4株假结核菌全基因序列进行比对，设计特异性的引物，对鼠疫菌、假结核菌和其他肠道细菌进行鉴定。结果用52株鼠疫菌、57株假结核菌和其他肠道菌株进行验证，结果显示，5对鼠疫菌的鉴定引物中，2对（PeI2和PeI11）仅在52株鼠疫菌中扩出目的条带，另3对引物（PeIl、PeI3和PeI12）除鼠疫菌外在部分假结核菌株中也扩出目的条带；5对假结核菌鉴定引物中，1对引物（Psi1）在52株鼠疫菌和57株假结核菌株中扩出目的条带，4对引物（Psi7、Psi16、Psi18和Psi19）仅在57株假结核菌株中均扩出目的条带，在鼠疫菌中未扩出目的条带。结论用鼠疫菌和假结核菌共有的Psil序列、鼠疫菌特有的PeI2和PeIll序列及假结核菌特有的Psi7、Psi16、Psi18和Psi19序列组成的基因鉴别方法，可以用于鼠疫菌和假结核菌的基因快速鉴别。Objective To establish a gene identification method of Yersinia pestis and Yersinia pseudotuberculosis for plague surveillance. Methods According to the specific genomic sequences of Y. pestis and Y. pseudotuberculosis, i.e. ＂pestis Island （PeI） ＂ and ＂pseudotuberculosis Island （Psi）＂ and the published genomic sequences of 12 strains of Y. pestis and 4 strains of Y. pseudotuberculosis, the specific identification primers of these sequences were designed. Results A total of 52 strains of Y. pestis and 57 strains of E pseudotuberculosis and other intestinal bacteria strains were tested with PCR. Of the 5 pairs of Y. pestis identification primers, PeI2 and PeIll were specific for Y. pestis. Besides Y. pestis, the primers PeI1, PeI3 and PeI12 could detect part of 57 E pseudotubereulosis strains. Of the 5 pairs of Y. pseudotuberculosis identification primers, Psi1 could detect all the 52 strains of Y. pestis and 57 strains of Y. pseudotuberculosis. Psi7, PsII6, PSI18 and Psll9 were specific for Y. pseudotuberculosis. Conclusion The primers Psi1, PeI 2 and PeIll, Psi7, Psi16, Psi18 and Psi19 can be used in the rapid identification of E pestis and E pseudotuberculosis, which can be also used to explore the circulation of atypical Y. pestis in quiescent plague foci.

关 键 词：鼠疫耶尔森菌 假结核耶尔森菌 基因 鉴别 

分 类 号：R440[医药卫生—诊断学]