桃红四物汤治疗兔早期筋膜间隔综合征的疗效观察及作用机制研究  

Experimental research on curative effect and mechanism of action of Taohong Siwu Tang( 桃红四物汤) for treatment of early compartment syndromes in rabbits

作　　者：赵佳盛 张耘[2,3] 

机构地区：[1]浙江省温岭市中医院,浙江温岭317502 [2]河南省洛阳正骨医院 [3]河南省骨科医院,河南洛阳471002

出　　处：《中医正骨》2015年第4期1-7,共7页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：河南省省属科研单位社会公益项目预研专项资金项目

摘　　要：目的:探讨桃红四物汤治疗早期筋膜间隔综合征的疗效及作用机制。方法:采用气压止血带压迫法将20只成年雄性新西兰大耳白兔制成后肢筋膜间隔综合征模型,造模后将20只兔子随机分为药物组和模型组,每组10只。药物组动物以桃红四物汤灌胃,每次7 m L;模型组以等量生理盐水灌胃;每天2次,连续1周。分别于造模前、造模后12 h、1 d、2 d、4 d、7 d观察动物的一般情况、小腿肿胀度、胫前间室内压及血液流变学指标。造模后7 d观察完成之后处死所有动物,取造模侧小腿胫前肌及腓深神经标本在光镜下检查。结果:1一般情况。在实验过程中模型组动物精神不振,饮食减少,大便正常,1只动物死亡;药物组动物精神、饮食尚可,大便稀溏,1只动物死亡。2小腿肿胀度。造模前后不同时间造模侧小腿肿胀度的差异有统计学意义,即存在时间效应(F=244.260,P=0.000)。2组造模侧小腿肿胀度比较总体上有统计学意义,即存在分组效应(F=128.651,P=0.000);除造模前和造模后12 h时外,其余各时点模型组的造模侧小腿肿胀度均高于药物组。时间因素与分组因素存在交互效应(F=39.453,P=0.000)。3胫前间室内压。造模前后不同时间造模侧胫前间室内压的差异有统计学意义,即存在时间效应(F=317.971,P=0.000)。2组胫前间室内压比较总体上有统计学意义,即存在分组效应(F=72.285,P=0.000);除造模前和造模后12 h时外,其余各时点模型组的胫前间室内压均高于药物组。时间因素与分组因素存在交互效应(F=31.071,P=0.000)。4血液流变学指标。造模前后不同时间全血黏度(200/S、30/S、5/S)的差异有统计学意义,即存在时间效应(F=420.316,P=0.000;F=977.477,P=0.000;F=3 283.000,P=0.000)。2组全血黏度(200/S、30/S、5/S)比较总体上有统计学意义,即存在分组效应(F=87.665,P=0.000;F=166.701,P=0.000;F=624.171,P=0.000);除造模前和造模后12 h时外,其余各时点�Objective：To study the curative effect and mechanism of action of Taohong Siwu Tang（桃红四物汤）for treatment of early compartment syndromes in rabbits.Methods：Twenty adult male big -eared New Zealand rabbits were made into compartment syndromes models in posterior limbs through compression with pneumatic tourniquet,and then the rabbits were randomly divided into drug group and model group,10 cases in each group.The rabbits in drug group were intragastric administrated with Taohong Siwu Tang（7 ml at a time）;while the others in model group were intragastric administrated with the same dose of normal saline,twice a day for 1 consecutive week.The general state of health,degree of leg swelling,anterior compartment pressure and hemorheology indexes of rabbits were observed before the modeling and at 12 hrs and 1,2,4 and 7 days after the modeling respectively.All the rabbits were executed at 7 days after the modeling,and their anterior tibial muscles and deep peroneal nerves were fetched out and sectioned for inspection under the light microscope.Results：During the experiment,the rabbits in model group presented with depressed spirit,decreased eating and normal stool,and 1 rabbit died.The rabbits in drug group presented with normal spirit,normal eating and sloppy stool,and 1 rabbit died.There was statistical difference in leg swelling degree between different timepoints,in other words,there was time effect（F =244.260,P =0.000）.There was statistical difference in leg swelling degree between the two groups in general,in other words,there was group effect（F =128.651,P =0.000）.The leg swelling degree of model group was higher than that of drug group except before the modeling and at 12 hrs after the modeling.There was interaction between time factor and group factor（F =39.453,P =0.000）.There was statistical difference in anterior compartment pressure between different timepoints,in other words,there was time effect（F =317.971,P =0.000）.There was statistical difference in anterior compartme

关 键 词：筋膜间隔综合征 桃红四物汤 血液流变学 动物实验 

分 类 号：R274.9[医药卫生—中西医结合]