调脂积对慢性应激致抑郁老年大鼠行为及NO、ET-1影响的研究  

Study on Tiaozhiji Formula on NO and ET- 1 and Chronic Stress- induced Behavioral Depression in Old Rats

作　　者：唐黎群[1,2] 余肖琴 潘智敏[4] 

机构地区：[1]浙江省人民医院,浙江杭州310014 [2]浙江中医药大学,浙江杭州310053 [3]杭州爱德医院,浙江杭州310004 [4]浙江省中医院,浙江杭州310014

出　　处：《中华中医药学刊》2015年第5期1162-1164,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省卫生厅项目(2012KYB017)

摘　　要：目的:通过观察老年抑郁大鼠模型的心血管活性物质一氧化氮、内皮素-1(NO、ET-1)变化,了解抑郁与心血管疾病的相关性;同时观察调脂积冲剂的可能干预作用。方法:Wistar老年大鼠共32只,随机分为正常空白组、模型对照组、调脂积冲剂干预组、氟西汀干预组。适应性喂养后,给予持续性温和刺激(包括冰水游泳、热刺激、禁食、禁水、夹尾、昼夜颠倒、高速水平摇晃、频闪灯照射)造抑郁模型。经检测造模成功后观察大鼠体质量变化、糖水偏嗜等一般情况,同时检测血管活性物质(NO、ET-1)水平。结果:给予持续性温和刺激56 d后,模型组与正常组比较,糖水偏嗜度有降低趋势,但无明显差异(P>0.05);调脂积冲剂组、氟西汀组与模型组比较,糖水偏嗜度有明显升高,其中调脂积冲剂组差异明显(P<0.05)。模型组与正常组比较,NO水平明显升高(P<0.05);调脂积冲剂组、氟西汀组与模型组比较,NO水平有下降趋势,其中调脂积冲剂组差异显著(P<0.05)。模型组与正常组比较,ET-1水平明显升高(P<0.05);调脂积冲剂组、氟西汀组与模型组比较,ET-1水平均呈下降趋势,氟西汀组差异明显(P<0.05)。结论:老年抑郁大鼠模型相关心血管活性物质(NO、ET-1)可产生变化,可能以此途径影响心血管疾病的发病及预后;调脂积冲剂对老年抑郁大鼠模型的行为及相关心血管活性物质具有干预作用。Objective ： By observing the geriatric depression rat model of cardiovascular active substances of nitric oxide and endothelin - 1 （ NO and ET - 1 ） change to understand the correlation between depression and cardiovascular disease. At the same time, to observe the effect of Tiaozhiji Formula. Methods ： 32 elderly Wistar rats were randomly divided into normal group, model control group, Tiaozhiji intervention group and fluoxetine intervention group. After feeding, continu- ous mild stimulations （including water swimming, thermal stimulation, fasting, ban water, tail clip, day and night upside down, high -speed horizontal shaking, flashing light irradiation） were used to make the model of depression. After the success of the model building, we observed weight of rats, partial addicted to sugar water and general situation and detec- ted the vascular active substances （ NO and ET - l ） levels. Results ： Supported by mild stimulation for 56 days, com- pared with normal group, model group had a tendency to reduce partial addicted to sugar water degree without obvious difference （ P 〉 0.05 ）. Tiaozhiji and fluoxetine groups compared with model group, the partial addicted to sugar water degree increased significantly and the Tiaozhiji group was more obvious （P 〈 0.05）. Compared with normal group, model groupg NO level increased significantly （ P 〈 0.05 ）. When Tiaozhiji and fluoxetine groups compared with model group, there was a downturn in the NO level and the Tiaozhiji group had significant difference （P 〈 0.05）. Compared with nor-mal group, model group ET 1 level increased significantly （P 〈 0.05 ）. When Tiaozhiji and fluoxetine groups compared with model group, tile level of ET - 1 had a downward trend and the fluoxetine group had significant difference （ P 〈 O. 05 ）. Conclusion ： The senile depression model rats related cardiovascular active substances （ NO and ET - 1 ） can change, which may affect the pathogenesis and prognosis of cardiovascular disease.

关 键 词：抑郁 NO ET-1 

分 类 号：R285.5[医药卫生—中药学]