脂氧素A4对脂多糖作用RAW264.7细胞的实验研究  

Preliminary study on effect of LXA4 on LPS-induced RAW264.7 Macrophages

作　　者：周虎[1] 黄利兴[2] 朱俊[3] 谢大泽 周南进[3,4] 

机构地区：[1]解放军第94医院,江西南昌330046 [2]赣南医学院第一附属医院,江西赣州341000 [3]南昌大学免疫与生物治疗研究所,江西南昌330006 [4]江西省医学科学研究院,江西南昌330006

出　　处：《临床军医杂志》2015年第4期336-338,341,共4页Clinical Journal of Medical Officers

基　　金：江西省教育厅项目(2012;2014;编号:Gjj1209;Gjj14096)

摘　　要：目的探讨脂氧素A4(LXA4)对脂多糖(LPS)作用巨噬细胞存活的影响作用。方法取对数生长期的鼠源性RAW264.7巨噬细胞株进行培养、传代。采用免疫印迹法(Western blot)检测不同浓度LPS处理巨噬细胞的pNF-κB p65蛋白水平,并应用CCK-8法观察LPS对巨噬细胞的毒性作用及LXA4对其存活率的影响。结果 LPS对巨噬细胞内pNF-κB p65蛋白水平存在影响,且在一定范围内呈剂量依赖关系;LXA4能够逆转LPS对巨噬细胞的毒性作用。结论 LXA4能够抑制LPS对巨噬细胞的作用及NF-κB的激活,从而减轻炎症反应。Objective To examine the effect of lipoxin A4 （LXA4） on the survival of macrophage.Methods Macrophages were treated with different concentrations of lipopolysaccharide （LPS） for 2-,4-and 6-h,respectively.The nuclear protein was isolated from the cells and the level of pNF-κB in macrophages was detected by Western Blot.Then the cells were cultured with or without 300 nM LXA4.Cytotoxicity was detected by CCK-8.We also observed the effect of LXA4 on the survival of macrophage,as well as its potential mechanism.Results LPS can promote the translocation of NF-κB in a concentration-dcpcndent way.LXA4 can reverse the cytotoxicity of LPS.Conclusion LXA4 may inhibit the cytotoxicity of LPS and the nuclear translocation of NF-κB,and thereby reduce the inflammation.

关 键 词：脂氧素A4 RAW264.7细胞 脂多糖 核转录因子 

分 类 号：R332[医药卫生—人体生理学]