创面分泌物培养前涂片镜检的临床意义  

Role of smear microscopic examination before bacterial culture

作　　者：黄芳[1] 林佩玲[1] 蔡媛媛[1] 王建福[1] 庄岳鹏[1] 

机构地区：[1]解放军第180医院检验科,福建泉州362000

出　　处：《临床军医杂志》2015年第4期406-408,共3页Clinical Journal of Medical Officers

摘　　要：目的探讨创面分泌物培养前涂片镜检对判断培养结果的意义。方法对150份创面分泌物标本作涂片革兰染色镜检,同时进行细菌培养。根据涂片结果将标本分为Ⅰ、Ⅱ和Ⅲ三类,并将涂片结果与培养结果进行比较分析。结果涂片合格标本(含Ⅰ和Ⅱ类)90例,涂片不合格标本(Ⅲ类)60例,标本合格率为60%;Ⅰ、Ⅱ和Ⅲ类标本培养细菌检出率分别为97.4%、40.4%和38.3%,合格标本的细菌总检出率(64.4%)显著高于不合格标本的检出率(P<0.05);Ⅰ、Ⅱ类标本涂片结果与培养结果的符合率分别为97.4%、65.4%,合格标本的总符合率为78.9%。Objective To investigate the role of smear microscopic examination before bacterial culture in the estimation of culture outcomes.Methods A total of 150 specimens from wound secretion were performed gram staining microscopy and bacterial culture.All the specimens were classified into three categories,i.e.category Ⅰ,Ⅱ and Ⅲ according to the results of smears,and then the results of smears were compared with that of culture.Results A total of 90 smear specimens were qualified （including category Ⅰ and Ⅱ） and 60 specimens were unqualified （category Ⅲ）,with a qualified rate of 60％.The positive rates of category Ⅰ,Ⅱ and Ⅲ culture specimens were 97.4％,40.4％ and 38.3％,respectively.The total detected rate of qualified specimens was 64.4％,significantly higher than that of the unqualified specimens （P ＜ 0.05）.The consistency rates of category Ⅰ and Ⅱ specimens between smears and culture were 97.4％ and 65.4％,and the total coincidence rate of qualified specimens was 78.9％.Conclusion Gram staining microscopic examination of wound secretion is valuable in improving the quality of specimens and the accuracy of pathogen culture.

关 键 词：创面分泌物 涂片 镜检 细菌培养 

分 类 号：R378[医药卫生—病原生物学]