大鼠脊神经后根中枢突切断对脊神经节卫星细胞表达Slit1的影响  

Expression of Slit1 in satellite glial cells of rat dorsal root ganglion after dorsal rhizotomy

作　　者：劳梅丽[1] 赵久红[1] 张显芳[1] 符碧薇[1] 张海英[1] 张全鹏[1] 刘辉[1] 易西南[1] 

机构地区：[1]海南医学院解剖学教研室,海南海口571159

出　　处：《海南医学院学报》2015年第6期721-723,727,共4页Journal of Hainan Medical University

基　　金：国家自然科学基金(81160158);海南省自然科学基金(8120000);海南医学院科研培育基金(HY2010-008);海南省教育厅科研项目(Hjkj2013-32)~~

摘　　要：目的:观察大鼠脊神经后根中枢突切断后相应背根神经节(DRG)卫星细胞(SGCs)Slit1的表达变化。方法:24只成年SD大鼠随机分为1、3、7d和14d组(n=6),脊神经L5、6左侧后根中枢突切断后,应用免疫组织化学方法检测各组相应DRG内卫星细胞Slit1表达变化。结果:DRG中枢突切断后1d组表达slit1阳性卫星细胞围绕的神经元比例开始增加,7d达到高峰,14d后逐渐下降,各组与对照组(右侧)比较有明显差异(P<0.01),各对照组间无明显差异(P>0.05)。对照组少量神经元slit1弱表达,1、3d组Slit1阳性的神经元比例分别为40.12%和59.45%,7、14d组神经元表达Slit1显著减少。结论:成年大鼠脊神经中枢突切断后,Slit1在SGCs和感觉神经元表达上调,SGCs源性Slit1可能参与了感觉神经损伤后修复过程。Objective：To observe changes of Slitl expression in satellite glial cells（SGCs） of rat dorsal root ganglion （DRG） after dorsal rhizotomy. Methods.. A total of 24 adult SD rats were randomly divided into 1 d,3 d,7 d and 14 d according to the survival time. After L5.6 dorsal rhizotomy, expression of Slit1 in the SGCs of DRG were detected respectively by immu- nohistochemical staining methods. Results= The rate of neurons surrounded by Slit-positive SGCs in ipsilateral DRG was in creased at 1 d after dorsal rhizotomy,reaching its peak at 7 d and decreased at 14 d. There was significant difference compared with control groups （right side,P〈0.01） ,but no difference among control groups （P〉0.05） . In 1 d,3 d groups ,the rate of Slit-positive-neurons in ipsilateral DRG was 40.12% and 59.45 %, respectively,the rate was significantly decreased in 7 d, 14 d groups, in contrast to slit1 weak expression in the control groups. Conclusions： The expression of Slitl in SGCs and neurons of DRG can he up-regulated by dorsal rhizotomy. Slitl from SGCs may participate in the process of regeneration after sensory nerve injury.

关 键 词：slit1 卫星细胞 脊神经节 免疫荧光双标 

分 类 号：R-332[医药卫生]