猪Zfx基因RNAi载体筛选及性控效果的观察  

Construction of RNAi Vecters of Swine Zfx Gene and Their Effects on Sexual Control

作　　者：宁孟影 孙丽荣 张永生[1] 贾斌[1] 秦炳燕 魏丽敏[1] 王绪海[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003 [2]内蒙古通辽市农畜产品质量安全中心,通辽028000

出　　处：《石河子大学学报（自然科学版）》2015年第2期192-195,共4页Journal of Shihezi University(Natural Science)

基　　金：新疆兵团博士基金项目(2012BB016);新疆兵团科技支疆项目(2014AB012);石河子大学动植物育种专项(GXJS2013-YZ07)

摘　　要：为了研究Zfx基因在精子发生过程中的作用,本研究利用RNA干扰技术针对猪Zfx基因m RNA设计3条si RNA并构建sh RNA干扰载体(p LL3.7/e、p LL3.7/f、p LL3.7/g)。对体外培养生精细胞进行干扰后,通过q RT-PCR测定Zfx基因m RNA表达丰度筛选出有效的干扰片段。然后利用有效的干扰载体进行睾丸注射,开展体内干扰试验。结果表明:构建的3个干扰载体,其中p LL3.7/e、p LL3.7/f对生精细胞Zfx基因的干扰率分别为44%(P<0.05)、56%(P<0.05),而p LL3.7/g对其无干扰效果(P>0.05);用p LL3.7/e、p LL3.7/f进行体内试验,结果显示后代仔猪雄性率率分别为51%(P>0.05),59%(P<0.05)。结论:Zfx基因干扰可以影响猪后代的性别比例,本研究可为今后进一步研究动物性别控制提供依据。To investigate the influence of offspring sex by silencing the Zfx gene during spermatogenesis in boar.Three recombinant expression vectors were constructed：pLL3.7/e,pLL3.7/f and pLL3.7/g.Then,recombinant expression vectors were transfected to spermatogenic cells in vitro,respectively.Expression level of Zfx mRNA was determined by qRT-PCR. Results showed that pLL3. 7/e and pLL3.7/f significantly inhibited the expression level of Zfx gene,and the inhibition rates were 44% （P〈0.05） and 56%（P〈0. 05）,respectively.pLL3.7/g had no inhibition （P〉0.05）.Thus,pLL3.7/e and pLL3.7/f were selected in vivo, and the respectively rate of offspring boar were 51%（P〉0.05） and 59%（P〈0.05）.Zfx gene interference can effectively control the offspring sex of large white, which provides the basis for further research on animal sex control.

关 键 词：Zfx基因 RNAI 猪 性别控制 

分 类 号：S828[农业科学—畜牧学]