小麦转录因子基因TaNF-YB2;1表达特征及遗传转化对植株抵御干旱和盐分逆境能力的影响  

Expression Patterns of Wheat Transcription Factor Gene Ta NF-YB2; 1 and Its Genetic Effects on Plant Tolerance to Stresses of Drought and Salt

作　　者：陈芳[1] 智一鸣 肖凯[1] 

机构地区：[1]河北农业大学农学院,河北保定071001

出　　处：《华北农学报》2015年第2期1-5,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31371618);河北省作物生长调控实验室项目

摘　　要：NF-YB型转录因子在介导植物抵御非生物逆境中发挥着重要作用。为揭示小麦该家族成员Ta NF-YB2;1在干旱和盐分逆境下的表达特征及超表达Ta NF-YB2;1对植株抵御上述逆境能力的影响。利用半定量RT-PCR技术,研究Ta NF-YB2;1的表达特征,采用农杆菌介导的遗传转化技术,建立了超表达Ta NF-YB2;1的转基因烟草植株。结果表明,Ta NF-YB2;1 c DNA全长序列为958 bp,编码163个氨基酸残基。在24 h PEG模拟干旱和盐分处理条件下,Ta NF-YB2;1的转录本数量明显上调。正常培养下,与野生型植株相比,转基因植株的生长和干物质积累量未发生改变;但在干旱和盐分处理下,转基因株系植株的生长较野生型植株明显改善,表现为植株个体体积增大,单株干物质积累量增多。因此,Ta NF-YB2;1的表达受到干旱和盐分逆境的明显诱导,通过对干旱和盐分逆境应答,该基因在介导植株抵御上述环境逆境中发挥着重要功能。NF-YB type transcription factor family plays critical roles in mediating plant tolerance to diverse abi-otic stresses. The purpose of this study was to understand the expression patterns of TaNF-YB2;1,a NF-YB tran-scription factor gene in wheat,and determine the function of this gene in regulating plant tolerance to aforemen-tioned stresses. The expression patterns of TaNF-YB2;1 were determined based on semiquantitative RT-PCR and transgenic tobacco plants overexpressing TaNF-YB2;1 were generated by adopting the Agrobacteriun-mediated ge-netic transformation approach. The results indicated that TaNF-YB2;1 had a cDNA full length of 958 bp,encoded a 163 amino acid-polypeptide. Under the conditions of drought and salt stress,the transcripts of TaNF-YB2;1 were drastically upregualted in comparison those under the condition of normal growth,suggested that it responds to a-bove stressors. Under normal growth,the growth features and dry mass of the plants overexpressing TaNF-YB2;1 were similar to wild type. However,the plants overexpressing TaNF-YB2;1 exhibited significantly improved growth features and dry mass compared with the wild type plants under treatments of drought and salt. Therefore,the ex-pression of TaNF-YB2;1 was dramatically induced by stresses of drought and high salinity. TaNF-YB2;1 acts as a critical regulator in mediating plant tolerance to drought and salt through its response to above stressors.

关 键 词：小麦 转录因子基因TaNF-YB2 1 表达 旱盐胁迫 抵御 

分 类 号：S512.03[农业科学—作物学] Q78[生物学—分子生物学]