白粉菌诱导的番茄叶片AD-cDNA文库的构建及初步分析  

Construction and Primary Analysis of Tomato Leaf AD-cDNA Library Induced by Powdery Mildew Fungus

作　　者：刘严[1,2] 张怡[2] 康静敏[1,2] 刘柯[2] 张超[2] 李鹏坤[2] 安一博 周晗[2] 黄辉[2] 李成伟[2] 

机构地区：[1]郑州大学生命科学学院,河南郑州450001 [2]周口师范学院植物遗传与分子育种重点实验室,河南周口466001

出　　处：《华北农学报》2015年第2期47-50,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31071807;31272168)

摘　　要：为了研究番茄白粉菌与番茄相互作用的分子机制，以白粉菌诱导的番茄叶片为材料，利用 SMART 技术构建了番茄的 AD-cDNA文库。该文库总库容量为3.77×1010 cfu，重组率达到96%，插入片段长400~2000 bp，平均长度约为1000 bp。随机挑取50个克隆进行测序，利用生物信息学软件进行初步分析，表明获得的文库质量较高。In order to study the interaction between powdery mildew fungus and tomato,the AD-cDNA library of tomato leaves infected by Oidiun neolycopersici was constructed using SMART technology. The titer of the con-structed cDNA library was 3. 77 × 1010 cfu,and the recombination rate of the library was about 96%,the insert frag-ments ranged from 400 bp to 2 000 bp,and the average length of the library was about 1 000 bp. Fifty clones were randomly picked from the library for sequencing and functional analysis. The results indicated that the library was qualified for the study on the interaction between powdery mildew fungus and tomato.

关 键 词：番茄 番茄白粉菌 SMART技术 AD-cDNA文库 

分 类 号：Q78[生物学—分子生物学] S436.412[农业科学—农业昆虫与害虫防治]