大丽轮枝菌酵母双杂交cDNA文库的构建  

Construction of Yeast Two-hybrid cDNA Library of Verticillium dahliae

作　　者：康静敏[1,2] 刘坤[2] 刘严[1,2] 张怡[2] 李成伟[2] 谭光轩[2] 

机构地区：[1]郑州大学生命科学院,河南郑州450001 [2]周口师范学院植物遗传与分子育种重点实验室,河南周口466001

出　　处：《华北农学报》2015年第2期51-54,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31440076;31272168)

摘　　要：为了分离克隆与植物互作的大丽轮枝菌致病相关蛋白基因，利用 SMART 技术构建了大丽轮枝菌酵母双杂交 cDNA 文库。结果表明，构建的文库滴度为5.2×107 cfu/mL，库容为3.9×107 cfu；文库 cDNA 插入片段长度主要分布在0.5~2.0 kb，平均为1 kb；文库重组率为96%。表明文库质量较好，为筛选与植物互作的大丽轮枝菌致病蛋白基因奠定了基础。To seek out the V. dahliae proteins involved in the interaction of plant with V. dahliae,a yeast two- hybrid library of V. dahliae was constructed using SMART technique. Results showed that the titer of cDNA library was 5.2 × 107 cfu/mL, the library contained 3.9 × 107 cfu independent clones, the size distribution of insert fragments ranged from 0.5 -2.0 kb,and the recombination rate was about 96%. These data demonstrated that the library could meet the requirements of standard cDNA library,which laid a foundation for screening the interaction proteins from V. dahliae.

关 键 词：黄萎病 大丽轮枝菌 酵母双杂交文库 SMART技术 

分 类 号：S432.1[农业科学—植物病理学] Q78[农业科学—农业昆虫与害虫防治]