大白菜抗根肿病基因位点CRa和CRb的分子标记鉴定  

Identification of CRa and CRb Molecular Markers of Clubroot Resistance Gene in Chinese Cabbage

作　　者：杨征[1,2] 杨晓云[2] 张清霞[2] 司朝光[2] 张淑霞[2] 王媛[2] 

机构地区：[1]青岛农业大学园艺学院,山东青岛266109 [2]青岛市农业科学研究院,山东青岛266100

出　　处：《华北农学报》2015年第2期87-92,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家科技支撑计划项目(2012BAD02B01-11);青岛市公共领域科技支撑计划项目(12-1-3-18-nsh);山东省现代农业产业技术体系创新团队任务

摘　　要：利用大白菜抗根肿病基因CRa和CRb分子标记(SC2930和KBr H129J18R)引物组,对78份大白菜材料进行抗根肿病分子标记鉴定。结果表明,在这78份材料中,有34份材料含有SC2930-T(CRa抗病标记)标记,其中杂合抗病位点材料17份,纯合抗病位点材料17份。有37份材料含有KBr H129J18R抗病标记,其中纯合抗病位点材料15份,杂合抗病位点材料22份。有20份材料不含有CRa和CRb所对应的抗病标记,23份材料含有2个抗病标记。该研究初步明确了78份参试大白菜材料所含抗根肿病基因CRa和CRb类型,为大白菜抗根肿病育种提供材料选择依据。The objective of this study was to use 2 gene molecular markers of clubroot resistance from Chinese cabbage（SC2930 and KBrH129J18R）,to identify clubroot resistance genes in 78 resources of Chinese cabbage. The results showed that there were 34 resources with SC2930 resistance marker in 78 resources. Including 17 heterozygous loci materials and 17 homozygous loci materials. 37 resources had KBrH129J18R resistance marker. Including 22 het-erozygous loci materials and 15 homozygous loci materials. 20 resources hadn′t the resistance markers of CRa or CRb. 23 resources had both 2 resistance markers. The study defined the clubroot resistance gene types of CRa and CRb in the 78 resources,and provided a material basis of breeding for clubroot resistance in Chinese cabbage.

关 键 词：大白菜 根肿病 抗病基因 分子标记 

分 类 号：Q78[生物学—分子生物学] S634.03[农业科学—蔬菜学]