大白菜育性相关蛋白差异表达  

Differencial Expression of Fertility-related Proteins in Chinese Cabbage

作　　者：毕青[1] 周雪[1] 冀瑞琴[1] 冯辉[1] 

机构地区：[1]沈阳农业大学园艺学院,辽宁沈阳110866

出　　处：《华北农学报》2015年第2期128-131,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(312721-57);辽宁省自然科学基金项目(2013020071)

摘　　要：为揭示复等位基因遗传的大白菜雄性不育分子机制,通过基于质谱的蛋白质组学同位素相对标记与绝对定量技术技术(i TRAQ),开展了大白菜核基因雄性不育蛋白质组学研究,以找到不育与可育材料中差异表达蛋白,从蛋白水平来进一步揭示大白菜雄性不育的分子遗传机制。通过研究,共发现了358个差异蛋白,其中可育中上调表达的蛋白有226个,下调表达的蛋白有132个,GO分析结果表明,鉴定的蛋白质组数据具有较好的生物学功能覆盖范围。通过双向电泳验证,差异蛋白点差异特性与i TRAQ结果类似,表明i TRAQ用于差异蛋白分析结果可靠。To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage（ Brassica rapa L. ssp pekinensis）,the iTRAQ quantitation technique based on mass spectrometry were used to study the differencial expression between proteins fertile- and sterile-buds. This research would provide the basis for analyzing the molecular mechanism of multiple-allele-inherited male sterility in Chinese cabbage. In this re-search,a total of 358 differencial expressed proteins（226 up-expressed and 132 down-expressed proteins in fertile buds）were found and Gene Ontology（ GO）analysis showed that the proteins identified data has better coverage of biological functions. It is verified by two-dimensional electrophoresis（2-DE）. This results is consistent with iTRAQ technique,which indicated that using iTRAQ to analyze protein profile is reliable.

关 键 词：大白菜 核基因雄性不育 ITRAQ 差异表达蛋白 

分 类 号：Q78[生物学—分子生物学] S634.03[农业科学—蔬菜学]