39个中药材提取物对Aβ_(25-35)诱导SH-SY5Y细胞损伤的保护作用筛选研究  

Screening of 39 Chinese Medicinal Extracts on Protective Effect of Aβ_(25-35)- induced SH-SY5Y Cell Injury

作　　者：陶晓倩[1,2] 胡玉梅[1,2] 王俨如 李娜[1,2] 曹亮[1,2] 孟兆青[1,2] 丁岗[1,2,3] 王振中[1,2,3] 萧伟[1,2,3] 

机构地区：[1]江苏康缘药业股份有限公司,连云港222001 [2]中药制药过程新技术国家重点实验室,连云港222001 [3]江苏省企业院士工作站,连云港222001

出　　处：《世界科学技术-中医药现代化》2015年第1期61-65,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重大新药创制专项(2013ZX09402203):现代中药创新集群与数字制药技术平台;负责人:王振中

摘　　要：目的:以SH-SY5Y细胞作为神经元代表,建立β淀粉样蛋白(Aβ_(25-35))诱导细胞损伤的阿尔茨海默病(Alzheimer's Disease,AD)体外细胞筛选模型,并将其应用于筛选39个中药材提取物中的杭神经细胞损伤活性组分。方法:采用CCK-8法进行活性初筛,CCK-8法和Hoechst33342/PI双染色分析法复筛活性组分。结果:初筛和复筛结果一致表明,39个药材提取物中黄柏40%乙醇部位(HB40)和95%乙醇部位(HB95)有较好的抗Aβ损伤活性。结论:提示黄柏有潜在的治疗杭阿尔茨海默病的药用价值。The model ofAβ25-35-induced SH-SY5Y （human neuroblastoma cell） injury was selected to screen neuroprotective compounds from 39 Chinese medicinal extracts. The CCK-8 assay was used for preliminary screening; and Hoechst 33342/PI double staining method was used for validation. The results showed that among 39 Chinese medicinal extracts, HB40 and HB95 had obviously protective effects onAβ25-35-induced SH-SY5Y injury. It was concluded that Huang-Bai had potential medicinal value in Alzheimer＇s disease treatment.

关 键 词：SH-SY5Y细胞 AΒ25-35 阿尔茨海默病 黄柏 

分 类 号：R285.5[医药卫生—中药学]