“脊髓康”对大鼠脊髓损伤区Nogo-A表达的影响  

Effect of Ji-Sui-Kang on Expression of Nogo-A in Spinal Cord Injury Rats

作　　者：杨俊锋[1] 马勇[1] 张亚峰[2] 陈剑峰[2] 华臻[1] 王建伟[2] 

机构地区：[1]南京中医药大学,南京210023 [2]无锡市中医医院南京中医药大学骨伤研究所,无锡214000

出　　处：《世界科学技术-中医药现代化》2015年第1期89-95,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：江苏省科技厅自然科学基金项目(BK2011180):"脊髓康"调节Nogo-Ng R信号通路促进神经再生作用的实验研究;负责人:王建伟;江苏省教育厅2013年度普通高校研究生科研创新计划项目(CXZZ13-0614):"脊髓康"干预Nogo-Ng R信号通路调节轴突再生的实验研究;负责人:杨俊锋

摘　　要：目的:观察中药"脊髓康"对大鼠脊髓急性损伤后脊髓组织Nogo-A表达的影响。方法:选用162只SD大鼠,随机分成假手术组、模型组、强的松组、脊髓康高剂量组、脊髓康中剂量组、脊髓康低剂量组,每组27只,分别于干预后3天、7天、14天处死大鼠,以免疫组化、Western Blot及荧光定量PCR检测大鼠脊髓组织中Nogo-A的表达量。结果:脊髓损伤各组在损伤后3天时Nogo-A蛋白和m RNA表达较低,7天后迅速上升达到高峰,至14天逐渐下降。与模型组比较,脊髓康中剂量组和强的松组在损伤后3天、7天、14天时,Nogo-A蛋白和m RNA表达差异均有统计学意义(P<0.05)。结论:中药"脊髓康"能有效抑制大鼠脊髓损伤区Nogo-A表达,有利于脊髓损伤修复。This study was aimed to observe the effect of Chinese medicine di-Sui-Kang （JSK） on the expression of Nogo-A in acute spinal cord injury rats. A total of 162 SD rats were randomly divided into the sham-operated group, model group, prednisone group, JSK group of high, middle and low dose, with 27 rats in each group. The rats were sacrificed on the 3^rd, 7^th and 14^th day after intervention, respectively. The expression of Nogo-A of spinal cord tissues among all rats was detected by immunohistochemistry, western blot and fluorescence quantitative PCR. The results showed that Nogo-A protein and mRNA expression of spinal cord injury rats were decreased on the 3^rd day after injury. However, it increased rapidly to the climax on the 7^th day. And then, it gradually decreased on the 14^th day. Compared with the model group, there were statistical differences on Nogo-A protein and mRNA expression of rats in the middle dose JSK group and prednisone group on the 3^rd, 7^th and 14^th day after injury （P〈0.05）. It was concluded that Chinese medicine JSK can effectively inhibit the expression of Nogo-A in acute spinal cord injury rats. It promoted the nerve regeneration in the spinal cord.

关 键 词：脊髓康 脊髓损伤 NOGO-A 神经再生 大鼠 

分 类 号：R651.2[医药卫生—外科学]