补肾法对再生障碍性贫血疗效及对Th17/Treg相关转录因子水平的影响  

Effect of the Tonifying Kidney Treatment Method on the Peripheral Levels of Th17 /Treg Cell-Associated Transcription Factors in Aplastic Anemia

作　　者：胡令彦[1] 胡明辉[1] 周永明[1] 陈英坤[2] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院血液科,上海200337 [2]浙江省杭州市萧山区第一人民医院血液科,浙江杭州311200

出　　处：《云南中医学院学报》2015年第2期1-5,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81001510);国家中医药管理局中医临床研究基地业务建设科研专项基金(JDZX2012179)

摘　　要：目的研究补肾法对再生障碍性贫血疗效及对Th17/Treg相关转录因子水平的影响。方法收集91例再生障碍性贫血患者为治疗组,辨证分为肾阴虚组,肾阳虚组,肾阴阳两虚组,用补肾方治疗。30例为对照组。统计2组有效率差异。同时采用RT-PCR方法检测2组治疗前后FOXP3m RNA,IL-17Am RNA,ROR-γtm RNA水平并比较。结果中医证候疗效治疗组优于对照组。治疗组ROR-γt m RNA治疗前高于治疗后,FOXP3 m RNA治疗前低于治疗后(有统计学意义)。对照组IL-17A m RNA治疗后低于治疗前。不同肾虚分型转录因子较治疗前明显变化,肾阴虚组IL-17A m RNA水平治疗后无明显下降。结论补肾法治疗再生障碍性贫血提高了中医证候有效率。补肾法疗效可能与干预Th17/Treg相关转录因子有关。Objective To investigate the effectiveness of the tonifying kidney treatment in those patients with aplastic anemia and the effect to the peripheral levels of Th17/Treg cell-associated transcription factors. Methods Collect 91 patients with CAA and divided them into the treatment group and control group. The treatment group was divided into SHENYINXU group, SHENYANGXU group and SHENYINYANGLIANGXU group treated them with method of tonifying kidney. 30 patients were divided to control group and count the effective rate. RT-PCR was used to test the serum levels of FOXP3mRNA, IL-17AmRNA, ROR-γ tmRNA Before and after treatment. Results After treatment, the effect of symptoms and signs of treatment group was better than that in the control group. After treatment, the level of ROR-γt mRNA In treatment group was lower than after treatment and the FOXP3 mRNA was higher, the level of IL-17a mRNA in control group decreaced after treatment. Th17 /Treg cell-associated transcription factors in different SHENXU groups varified after treatment. There was no significant decrease in The level of IL-17a mRNA in SHENYINXU group. Conclusion The method of BUSHEN in the CAA increased The effect of symptoms and signs. The effect of BUSHEN may rise by the Th17/Treg cell-associated transcription factors.

关 键 词：再生障碍性贫血 白细胞介素-17 维甲酸相关孤儿受体 叉头状转录因子3 补肾法 信使核糖核酸 

分 类 号：R285.6[医药卫生—中药学]