大鼠局灶性脑挫伤后p35及p25的表达变化  

Expression of p35 and p25 after Focal Cerebral Contusion in Rat

作　　者：王汉志[1,2] 李如波[1] 王正印[1] 张立军 

机构地区：[1]中国医科大学法医学院法医病理学研究室,辽宁沈阳110001 [2]苏州市公安局,江苏苏州215000 [3]调兵山市公安局,辽宁调兵山112700

出　　处：《法医学杂志》2015年第2期93-96,共4页Journal of Forensic Medicine

基　　金：辽宁省教育厅科学研究一般项目(L2013317);辽宁省自然科学基金资助项目(2014021027)

摘　　要：目的探讨大鼠局灶性脑挫伤后脑中p35和p25表达变化的时间规律性,为脑损伤时间推断提供更多依据。方法50只成年雄性SD大鼠随机分为损伤即刻(0h)组,伤后6h、12h、24h、3d、5d、7d、10d组,同时设立正常组和假手术组,每组5只,建立大鼠局灶性脑挫伤模型,运用HE、免疫组织化学染色及Western印迹法等方法检测损伤不同时间后损伤周边区脑组织p35及p25的蛋白表达。结果正常组和假手术组蛋白表达较多p35和少量p25。在局部脑挫伤后,p35随时间呈现单峰变化,p25随时间呈现双峰变化。结论大鼠局灶性脑挫伤后p35和p25的表达呈现不同规律性并有较好的时间相关性,可为脑损伤时间推断提供较好的依据。Objective To study the expression of p35 and p25 in rat after focal cerebral contusion and to provide experimental data for estimating brain injury time. Methods Fifty adult male SD rats were randomly divided into 0 h, 6 h, 12 h, 24 h, 3 d, 5 d, 7 d, 10 d after focal cerebral contusion, control and sham-operated groups （5 rats each group）. The focal cerebral contusion rat model was established. The expression of p35 and p25 protein of the damage peripheral zone in brain were detected by HEstain-ing, immunohistochemistry and western blotting at different injury time. Results Alarge number of p35 protein and a small amount of p25 protein were expressed in control group and sham-operated group. After focal cerebral contusion, p35 presented unimodal change with time and p25 presented bimodal changes with time. Conclusion Expression of p35 and p25 showed different regularity with good time correlation, which could help to estimate the brain injury time.

关 键 词：法医病理学 脑损伤 P35 p25 大鼠 

分 类 号：DF795.1[医药卫生—法医学]