婴幼儿脑干脑炎EV71-VP1、PSGL-1和SCARB2的表达  

Expression of EV71-VP1, PSGL-1 and SCARB2 in Tissues of Infants with Brain Stem Encephalitis

作　　者：李明[1] 孔小平[2] 刘宏[3] 程灵犀[4] 黄京璐[1] 权力[1] 午方宇 郝博[1] 刘超[3] 罗斌[1] 

机构地区：[1]中山大学中山医学院法医学系,广东广州510080 [2]广州市公安局番禺区分局,广东广州510180 [3]广州市刑事科学技术研究所,广东广州510030 [4]广州市公安局南沙区分局,广东广州510000

出　　处：《法医学杂志》2015年第2期97-101,104,共6页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(39700167);"十二五"国家重大科技攻关项目(2012BAK02B002);上海市法医学重点实验室开放课题资助项目(2013KF1307)

摘　　要：目的观察人肠道病毒71型(enterovirus 71,EV71)、P选择素糖蛋白配体1(P-selectin glycoprotein ligand-1,PSGL-1)和人清道夫受体B2(scavenger receptor B2,SCARB2)在婴幼儿脑干脑炎的表达,探究PSGL-1、SCARB2与EV71间的联系及EV71感染人体后可能的侵染途径和机制。方法收集EV71-VP1染色阳性的10例婴幼儿的器官、组织进行PSGL-1、SCARB2免疫组织化学染色,并与EV71-VP1免疫组织化学染色结果相比较。结果 EV71-VP1在脑内神经元、胶质细胞、血管周呈"袖套样"浸润的炎症细胞,胃底腺壁细胞呈强阳性;肺泡巨噬细胞,肠腺上皮细胞及黏膜层淋巴小结、腭扁桃体中淋巴细胞呈中度阳性;肠系膜淋巴结、脾的淋巴细胞中呈弱阳性。PSGL-1仅在胃底腺壁细胞,腭扁桃体隐窝鳞状上皮组织,肺泡巨噬细胞及各组织白细胞中表达。SCARB2除肠、脾组织外均有表达。结论 SCARB2与EV71组织分布具有相关性,在病毒的侵染及复制过程中起重要作用。胃可能是EV71复制的一个重要部位。Objective To understand the correlation of enterovirus 71 （EV71）, P-selectin glycoprotein ligand-1 （PSGL-1）, and scavenger receptor B2 （SCARB2） and to explore the possible pathway and mechanismof EV71 infection by observing the expression of EV71, PSGL-1 and SCARB2 in tissues of infants with brain stemencephalitis. Methods The organs and tissues of infants with EV71-VP1 positivity in their brain stems were chosen. Expression and distribution of EV71-VP1, PSGL-1, and SCARB2 were detected and compared by immunohistochemistry. Results Strong staining of EV71-VP1 was ob-served in the neuron, glial cells, the inflammatory cells of perivascular cuffing, parietal cells of the gas-tric fundus gland while alveolar macrophages, intestinal gland epitheliumcells, mucosa lymphoid nodule and lymphocyte of palatine tonsil showed moderate staining and weak staining were displayed in mesen-teric lymph nodes and lymphocyte of spleen. PSGL-1 expression was detected in parietal cells of the gastric fundus gland, tonsillar crypt squamous epithelium, alveolar macrophages and leukocytes in each tissue. SCARB2 expression was observed in all the above tissues except the intestines and spleen. Con-clusion T he distribution of EV71 correlates with SCARB2 expression. SCARB2 plays an important role in virus infection and replication. Stomach may be an important site for EV71 replication.

关 键 词：法医病理学 脑炎 肠道病毒属 P-选择素糖蛋白配体1 清道夫受体 B类 婴幼儿 

分 类 号：DF795.1[医药卫生—法医学]