湖南省猪源粪肠球菌耐药性分析  

DRUG RESISTANCE OF SWINE ENTEROCOCCUS FAECALIS IN HUNAN PROVINCE

作　　者：王送林 李芸芳[1] 刘秋菊[1] 朱娜[1] 蒋伟[1] 邓治邦[1] 黎满香[1] 

机构地区：[1]湖南农业大学动物医学院,长沙410128

出　　处：《中国动物传染病学报》2015年第2期41-46,共6页Chinese Journal of Animal Infectious Diseases

基　　金：湖南省科技计划项目(2013FJ6060)

摘　　要：为了解湖南省猪源粪肠球菌临床分离株的耐药性及各耐药基因的分布情况,使用K-B法检测了42株猪源粪肠球菌对11种抗生素的敏感性,采用PCR方法检测了粪肠球菌中8种耐药基因的分布情况。结果显示,湖南省临床分离的42株粪肠球菌对大部分的抗生素高度耐药,对四环素、氯霉素、苯唑青霉素、红霉素、米诺霉素、左氧氟沙星、高浓度庆大霉、高浓度链霉素、环丙沙星、万古霉素、青霉素G的耐药率依次为100%、92.9%、88.1%、83.3%、81%、57.1%、52.4%、47.6%、45.2%、31%、11.9%;耐药基因的检出率为Aac(6)'/aph(2'')97.6%、ant(6)'-Ⅰ90%、aph(3)'-Ⅲ76.2%、tet M 90.5%、erm B 73.8%、Van A 4.8%、Van B 54.8%、Van C 88.1%。从表型与基因型共同分析粪肠球菌的耐药性,发现粪肠球菌的多耐药性严重,其耐药表型与耐药基因并不完全一致。The aim of this study was to determine the drug resistance ofEnterococcus faecalis and the genes related to resistance. Total 42 isolates were evaluated for their sensitivity to 11 antimicrobial drugs by K-B disc diffusion method and for the presence of 8 resistance genes in PCR. All 42 isolates ofEnterococcus faecalis were highly resistant to most antimicrobial drugs. The drug resistance percentages of these isolates to aeheomycin, chloramphenicol, oxacillin, erythromycin, minocycline, levofloxacin, gentamiein, streptomycin, ciprofloxacin, vancomycin and penicillin G were 100%, 92.9%, 88.1%, 83.3%, 81%, 57.1%, 52.4%, 47.6%, 45.2%, 31% and 11.9%, respectively. The percentages of drug resistance genes were Aac（6）＇/aph（2＂）97.6%, ant（6）＇- Ⅰ 90%, aph（3）＇- Ⅲ 76.2%, terM90.5%, ermB 73.8%, VanA 4.8%, VanB 54.8% and VanC 88.1%, respectively. In addition, analysis of multi-drug resistance ofEnterococcus faecalis did not find relationship between phenotypes and resistance genes.

关 键 词：粪肠球菌 耐药性 耐药基因 

分 类 号：S852.611[农业科学—基础兽医学]