硒胁迫下彩叶草主要化学组分的FTIR和PXRD分析  

FTIR and PXRD Analysis on Chemical Component of Coleus blumei to SeleniumStress

作　　者：袁菊红[1] 胡绵好[2] 

机构地区：[1]江西财经大学艺术学院 [2]江西财经大学鄱阳湖生态经济研究院,南昌330032

出　　处：《上海交通大学学报（农业科学版）》2015年第2期39-46,共8页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金项目(21407069;21367013;41001376);江西财经大学优秀青年学术人才支持计划项目(K00292025)

摘　　要：为了阐明彩叶草（Coleus blumei Benth.）对硒（Se）胁迫的耐性机理,采用傅里叶变换红外光谱法（FTIR）和粉末X射线衍射法（PXRD）研究在不同Se浓度（0、0.1、0.5、1.0、2.5、5.0mg/L）处理下彩叶草根、茎、叶化学组分及其结构的变化。结果表明,不同浓度Se处理下彩叶草各器官中化学组分的FTIR峰形基本不变,只有某些参与重金属吸收、转运及其积累的官能团如羟基（3 360～3 906cm^-1）、羧基（1 321～1 333cm^-1）、酰胺基（1 611～1 646cm^-1）、芳烃的C-H基（800～840cm^-1）及其C-Cl（618～706cm^-1）等的透射峰发生了不同程度的位移。彩叶草各器官中与植物生理有关的化学成分的特征峰大多数随Se浓度变化而变化。说明这些基团与Se的结合密切相关,进一步解释了彩叶草的耐Se及其在各器官积累Se的机理。不同浓度Se处理的彩叶草各器官物相结构主要组分以非晶体物质组分为主且均含有晶体成分,但处理浓度高低影响了其晶体物质组分含量的多少。This study aimed to elucidate the mechanism of Coleus blumei Benth to selenium（Se）tolerance.The changes of chemical composition and its structure in the roots,stems and leaves of C.blumei treated with different concentrations of Se were investigated using Fourier transform infrared spectroscopy（FTIR）and Powder X-ray diffraction（PXRD）technology.The peak shape of chemical constituents in each organ of C.blumei remained invariable basically with different concentrations of Se treatments,except certain transmission peak shifts of some functional groups such as hydroxy groups（3 360～3 906cm^-1）,carboxyl groups（1 321～1 333cm^-1）,acidamide groups（1 611～1 646cm^-1）,C-H of aromatic hydrocarbons（800～840cm^-1）,and C-Cl of aromatic hydrocarbons（618～706cm^-1）,which are involved in the process of absorption,transport and accumulation of Se.The characteristic peak on each organ of C.blumei connectingwith the chemical constituents of plant physiology changed with Se concentration.It is suggested that these groups were referred in binding Se,and may further explain the mechanism of C.blumei resistance to Se and its accumulated in different organs.The phase structure in each organ of C.blumei treated with different concentrations of Se were given priority to amorphous material components and contained crystal composition,but the contents of crystal components were influenced by Se concentration level.

关 键 词：彩叶草 硒胁迫 傅里叶变换红外光谱法(FTIR) 粉末X射线衍射分析(PXRD) 

分 类 号：X53[环境科学与工程—环境工程]