补肾养血法对超促排卵未孕大鼠子宫内膜微环境的影响  

Effects of “tonifying kidney and nourishing blood method”on microenvironment of uterus in non-pregnant rats with ovarian hyperstimulation

作　　者：于妍妍[1] 贡欣[1] 金哲[1] 刘艳霞[1] 

机构地区：[1]北京中医药大学东方医院妇科,北京100078

出　　处：《北京中医药》2015年第4期276-279,F0003,共5页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(811002612)

摘　　要：目的探讨中医补肾养血法对超促排卵未孕大鼠子宫内膜微环境的影响。方法建立大鼠超促排卵模型,以补肾养血方干预未孕大鼠,分析其子宫内膜形态、微血管密度、子宫容受性分子标志物的改变。结果超促排卵可减小子宫内膜厚度,增加腺上皮高度使腺体密集,降低白血病抑制因子(LIF)蛋白及整合素α4蛋白的表达,改变子宫内膜微环境;补肾养血法可改善未孕超促排卵大鼠子宫内膜形态,升高整合素α4和LIF表达水平。结论补肾养血法可以改善子宫内膜微环境,提高子宫内膜容受性,从而保证早期的妊娠稳定。Objective To explore the effects of ＂tonifying kidney and nourishing blood method＂ on microenvironment of uterus in non-pregnant rats with ovarian hyperstimulation. Methods The rat model of ovarian hyperstimulation was established. The ＂tonifying kidney and nourishing blood method＂ was used in non-pregnant rats, and the endometrial morphology, mierovessel density, markers of receptivity of the uterus moleeular were analyzed. Results The ovarian hyperstimulation could reduce endometrial thickness, increase glandular epithelium, leading to dense glands; the expression of leukaemia inhibitory factor （LIF） and integrin α4 protein was reduced; the microenvironment of endometrium was changed. However, ＂tonifying kidney and nourishing blood method＂ could improve the endometrial morphology in non-pregnant rats with ovarian hyperstimulation, increase microvessel density and increase the expression of integrin or4 and LIF. Conchmions The ＂tonifying kidney and nourishing blood method＂ can improve microenvironment of endometrium, enhance the endometrial receptivity in order to maintain stability of pregnancy at early stage.

关 键 词：不孕症 超促排卵 补肾养血法 子宫内膜容受性 大鼠 

分 类 号：R271.14[医药卫生—中医妇科学]