淫羊藿苷对前成骨细胞株OCT1细胞BMP-2 mRNA、Runx-2 mRNA表达的影响  

Effects of Icariin on expressions of BMP-2 mRNA and Runx-2 mRNA in preosteoblastic cell lines OCT1

作　　者：笪巍伟[1,2] 赵永见[1,2] 王拥军[1,2] 程少丹 施杞[1,2] 唐德志[1,2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海中医药大学脊柱病研究所,上海200032 [3]上海市光华中西医结合医院康复科,上海200052

出　　处：《上海中医药杂志》2015年第5期90-94,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：教育部创新团队发展计划项目(IRT1270);国家自然科学基金青年基金项目(81102604);上海市卫生系统新优青培养计划项目(XYQ2013085);上海市科委青年科技启明星计划项目(14QA1403500);上海市教委科研创新项目(14YZ051);上海高校青年教师培养资助计划项目(ZZszy13005)

摘　　要：目的探讨淫羊藿苷对成骨细胞增殖和分化的影响及作用机制。方法常规培养前期实验建立的前成骨细胞株OCT-1细胞(OCT1细胞),采用淫羊藿苷进行干预48 h,浓度分别为10 mg/L、30 mg/L及60 mg/L,并以BMP2纯化蛋白(50μg/L)为阳性对照。利用MTT法检测成骨细胞增殖率,实时荧光定量RT-PCR检测成骨细胞中BMP2 mRNA、Runx2 mRNA的表达。结果与正常对照组比较,淫羊藿苷能明显促进成骨细胞的增殖(P<0.05);淫羊藿苷可显著提高成骨细胞中BMP2 mRNA、Runx2 mRNA表达量(P<0.05)。结论淫羊藿苷通过激活前成骨细胞中BMP信号通路上调Runx2的表达,从而诱导成骨细胞的增殖和分化。Objective To observe the effect and mechanism of Icariin on proliferation and differentiation of osteoblasts. Methods The preosteoblastic cell lines OCTI cells were cultured under the routine method, then iutervened for 48 hours with Icariin, at the concentration of 10ug/mL, 30ug/mL and 60ug/mL respectively, and the purified protein BMP2 （50μg/L） as a positive control. MTT method was performed to detect the proliferation rate of osteoblasts, and RT-PCR analysis was performed to observe the expression of BMP2 mRNA, Runx2 mRNA. Results Compared with the normal control group, Icariin promoted the osteoblast proliferation obviously （ P 〈 0.05 ） ; Icariin increased the expression of BMP2 mRNA and Runx2 mRNA in osteoblasts significantly （ P 〈 0.05 ）. Conclusion Icariin can induce the proliferation and differentiation of osteoblasts by activating the BMP signaling pathway and up-regulating the expression of Runx2 in preosteoblastie cell lines.

关 键 词：细胞株 成骨细胞 淫羊藿苷 细胞增殖 实时荧光定量PCR 

分 类 号：R285[医药卫生—中药学]