降铅Ⅰ号对低水平铅暴露仔鼠脑细胞凋亡的影响  

Effect of “Jiangqian I ” on brain apoptosis of the offspring of rats exposed to low level lead exposure

作　　者：郑月琳[1,2] 冯建华[3] 陈玉燕[2] 

机构地区：[1]浙江大学研究生院,浙江杭州310027 [2]浙江省中医院儿科,浙江杭州310000 [3]浙江大学医学院附属第二医院儿科,浙江杭州310009

出　　处：《上海中医药杂志》2015年第5期99-101,共3页Shanghai Journal of Traditional Chinese Medicine

基　　金：浙江省教育厅科研项目(20050858)

摘　　要：目的探讨降铅Ⅰ号对低水平铅暴露仔鼠脑细胞凋亡的干预作用及神经行为改善的机制。方法成年Wistar大鼠按雌∶雄=2∶1比例交配后,随机分为正常组和实验组。从孕期第1天起,分别饮用蒸馏水和0.2%醋酸铅溶液,直至第21天仔鼠断乳,断乳仔鼠用于实验。实验组仔鼠随机分为铅模型组、EDTA对照组、降铅Ⅰ号低剂量组、降铅Ⅰ号中剂量组、降铅Ⅰ号高剂量组。正常组仔鼠作为空白组。各组给予相应干预。采用原子吸收分光光度石墨炉法测定血铅和脑铅含量,用Y迷宫观察用药后对仔鼠学习和记忆能力的影响。结果治疗后各治疗组仔鼠的血铅水平均显著低于铅模型组(P<0.001);EDTA组、降铅Ⅰ号低剂量组的脑铅水平与铅模型组比较明显下降(P<0.05),降铅Ⅰ号各组的脑铅水平与EDTA组比较无差异(P>0.05);降铅Ⅰ号低剂量组的记忆保持率与空白组比较差异无统计学意义(P>0.05)。降铅Ⅰ号低剂量组、降铅Ⅰ号高剂量组的记忆保持率高于铅模型组(P<0.05,P<0.001)。降铅Ⅰ号低剂量组的记忆保持率高于EDTA组(P<0.01),降铅Ⅰ号低剂量组的记忆保持率高于降铅Ⅰ号中剂量组(P<0.05);各治疗组较铅模型组海马凋亡标记指数均显著下降,差异显著(P<0.05,P<0.01)。结论降铅Ⅰ号能降低低水平铅暴露仔鼠的血铅和脑铅含量,明显改善低水平铅暴露仔鼠的学习记忆能力。Objective To observe the intervention effect of ＂ Jiangqian I ＂ on brain apoptosis and mechanism study on improvment of neurobehavior. Methods Adult Wistar rats mated by female：male =2 ： 1 ratio were randomly divided into normal group and the experimental group which were given distilled water or 0.2% lead acetate solution respectively from the 1st day after pregnancy until the 21 days when the offspring began to be weaned. Weaning offspring is used for the experiment. Experimental rats were randomly divided into lead-exposed model group, EDTA group, the low dose ＂Jiangqian I＂ group, the middle dose ＂Jiangqian I＂ group and the high dose ＂Jiangqian I＂ group. The normal group used as blank group. Their blood and brain of lead was determined by atomic absorption spectrophotometry. Effects of learning and memory capacity of offspring after the medication were observed with Y-maze and apoptotic cells condition was detected with SP immunohistochemical method. Results After treatment, the blood lead levels of lead-exposed model group was significantly decreased after detachment of Lead Pollution （ P 〈0. 001 ）. After treatment, the brain lead levels of EDTA group and the low dose ＂Jiangqian I＂ group were significantly lower than those of the model group （ P 〈 0.05 ）. There are no significant difference between the brain lead levels of ＂Jiangqian I＂ group and those of EDTA （ P 〉0.05 ） ; The rate of memory maintenance of the low and high dose ＂Jiangqian I＂ group were significantly higher than those of the model group （ P 〈0.05, P 〈0. 001 ）. The rate of memory maintenance of the low dose ＂Jiangqian I＂ group were significantly higher than those of the EDTA group （ P 〈0.01 ）. The rate of memory maintenance of the low dose ＂Jiangqian I＂ group were significantly higher than those of the middle group （ P 〈 0.05 ） ; the apoptosis index of each treatment group were significantly lower than those of the model group （ P 〈0.05, P 〈0.01 ）. Conclusion

关 键 词：降铅Ⅰ号 铅 凋亡 学习记忆能力 

分 类 号：R285.5[医药卫生—中药学]