芝麻素对自发性高血压大鼠心肌氧化应激损伤的保护作用  

Effect of sesamin on the protein i NOS and c-fos expression in myocardia of spontaneously hypertensive rats

作　　者：李伟[1] 杨解人[1] 李文星[1] 张俊秀[1] 唐丽娟[1] 杨慧[1] 熊莺[1] 

机构地区：[1]皖南医学院药理学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2015年第2期103-106,共4页Journal of Wannan Medical College

基　　金：安徽省自然科学基金项目(1308085QH145);安徽省高校青年教师科研资助计划(2008jq1151)

摘　　要：目的:观察芝麻素对自发性高血压大鼠(spontaneously hypertensive rat,SHR)心肌i NOS和c-fos蛋白表达来探讨其可能保护机制。方法:SHR 35只,随机分为SHR模型组、芝麻素[40、80、160 mg/(kg·d)]组、卡托普利[30 mg/(kg·d)]组及WKY阴性组,每天灌胃给药1次,连续16周。末次给药后,称体质量,HE染色观察心肌病理变化;化学比色法测心肌组织中T-SOD的含量;免疫组化法检测心肌诱导型一氧化氮合酶(inducible nitric oxide synthase,i NOS)和原癌基因(cellular oncogene fos,c-fos)阳性蛋白表达。结果:SHR组心肌细胞增生肥大且排列紊乱,组织中可见大量炎性细胞和脂肪组织浸润,心肌T-SOD的含量明显减少(P<0.01),心肌i NOS和c-fos蛋白表达显著增加(P<0.01),芝麻素和卡托普利组上述病理变化则有不同程度的改善;心肌T-SOD明显增加(P<0.01),i NOS和c-fos蛋白表达明显下调(P<0.01)。结论:芝麻素具有下调SHR心肌i NOS和c-fos蛋白表达,增强T-SOD能力,保护心肌损伤的作用。Objective：To observe the effects of sesamin on the protein iNOS and c-fos expression in myocardia of spontaneously hypertensive rats（ SHR）, and explore the potential protective mechanisms. Methods ： Thirty-five SHRs were randomly divided into group of SHR model, sesamin treatment in diverse dosage [ 40,80 and 160 rag/（ kg · d ） ] and captopril intervention [ 30 mg,/（ kg · d ） ] , and 7 Wistar-Kyoto rats （WKY） were included as negative controls. All rats were intragastrically administrated with the drugs once a day for consecutive 16 weeks. After the final intervention, the body weight （BW） was measured,and histopathological changes of the myocardia were observed by HE staining. Colorimetric analysis was performed to measure the contents of myocardial T-SOD, and immunohitochemical method was used to determine the expression of inducible nitric oxide synthase （iNOS） and c-los. Results： SHR group demonstrated hypertrophied myocardia with disordered arrangement. Large quantity of inflammatory cells and adipose tissue infiltration were seen in the myocardial tissues, and the expression of T-SOD was significantly reduced （ P 〈 0. 01 ）, whereas the protein iNOS and c-fos expression in myo- cardia was significantly up-regulated （P 〈 0. 01 ）. Myocardial pathological lesions were improved to a certain degree in the rats treated with sesamin and captopril, and T-SOD content was significantly increased （P 〈 O. 01 ）, whereas the expression of iNOS and c-fos was notably reduced in dose-dependent manner（ P 〈 0. 01 ）. Conclusion ： Sesamin could decrease the expression of iNOS and c-fos protein and enhance the ability of T-SOD, suggesting that it can be protective effect on myocardial injury in spontaneously hypertensive rats.

关 键 词：芝麻素 自发性高血压大鼠 心肌 T-SOD INOS C-FOS 

分 类 号：R285.5[医药卫生—中药学]