瑞舒伐他汀对大鼠缺血心肌嗜铬粒蛋白A和MR-proADM表达的影响  

Effect of rosuvastatin on expression of chromogranin A and original middle segment of MR-proADM in ischemia myocardial tissue of rats with acute myocardial infarction

作　　者：孙慎杰[1] 吴小鹏[1] 宋恒良[1] 李贵琦[1] 张娟[1] 万大国[1] 段温泉[1] 朱沙[2] 

机构地区：[1]郑州大学第二附属医院心内科,450014 [2]郑州大学基础医学院微生物学与免疫学教研室,450001

出　　处：《重庆医学》2015年第13期1741-1743,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81041102)

摘　　要：目的研究瑞舒伐他汀对急性心肌梗死大鼠缺血心肌嗜铬粒蛋白A(CGA)和肾上腺髓质素原中间片段(MRproADM)表达的影响。方法采用冠状动脉结扎术建立30只急性心肌梗死大鼠模型,分成对照组(n=15)和治疗组(n=15),以15只正常大鼠作为空白组,其中治疗组接受瑞舒伐他汀治疗。分别在手术治疗后3、7d和14d取5只大鼠的心肌组织。采用荧光定量PCR检测组织内CGA和MR-proADM mRNA转录量,采用Western blot检测组织内CGA和MR-proADM的表达量。结果治疗后第7天和第14天治疗组CGA和MR-proADM mRNA和蛋白表达量与空白组差异无统计学意义(P>0.05),对照组CGA和MR-proADM mRNA和蛋白表达量均显著高于治疗组和空白组(P<0.05)。结论瑞舒伐他汀可有效降低急性心肌梗死大鼠心肌组织内CGA和MR-proADM mRNA转录和蛋白表达。Objective To analyze the effect of rosuvastatin on the expression of chromogranin A and the original middle seg- ment of MR-proADM in ischemia myocardial tissue of rats with acute myocardial infarction, and to investigate its mechanism. Meth- otis The rat model with acute myocardial infarction was established with the coronary artery ligation. 30 model rats were divided into control group （n= 15） and the treatment group （n= 15） ,with 15 normal rats as the blank group. The treatment group received the rosuvastatin treatment. On 3,7,14 d after treatment,the myocardial tissues of 5 rats were taken. The fluorescence quantitative PCR was performed to detect chromogranin A and adrenomedullin original middle fragment of MR-proADM mRNA transcription, and Western blot was used to measure the expression of chromogranin A and adrenomedullin original middle segment of MR- proADM. Results On 7,14 d after treatmenr,CGA and MR-proADM mRNA and protein expression had no statistically significant difference between the treatment group and the blank group （P^0.05）,CGA and MR-proADM mRNA and protein expression of the control group were significantly high~ than those of the treatment group and the blank group （P^0.05）. Conclusion Rrosuv- astatin can effectively decrease the expression of chromogranin A,MR-proADM mRNA transcription and protein expression in the myocardial infarcted rats.

关 键 词：心肌梗死 嗜铬粒蛋白A 肾上腺髓质素原中间片段 瑞舒伐他汀 

分 类 号：R542.22[医药卫生—心血管疾病]