HDL影响冠心病患者血小板ox-LDL诱导活化的体外分析  被引量:7

Analysis in vitro of high-density lipoprotein affecting platelet activation induced by oxidized low-density lipoprotein in patients with coronary heart disease

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作  者:李运田[1] 杜大勇[1] 杨升华[1] 柳杨[1] 

机构地区:[1]中国人民解放军第三〇五医院心脏中心,北京100017

出  处:《重庆医学》2015年第13期1756-1758,共3页Chongqing medicine

基  金:全军保健科研基金项目(12BJZ29)

摘  要:目的探讨高密度脂蛋白(HDL)对氧化低密度脂蛋白(ox-LDL)诱导的人血小板活化的影响。方法将洗涤血小板按照等体积分为3组,Ⅰ组加入100μg/mL HDL;Ⅱ、Ⅲ组均加入PBS,3组均置于37℃环境下孵育15min。Ⅰ、Ⅱ组加入50μg/mL ox-LDL;Ⅲ组加入PBS,3组均在37℃再孵育15min。应用流式细胞仪检测3组CD62P表达水平,应用透射电镜观察3组血小板的形态结构。结果3组的血小板CD62P表达率差异有统计学意义(P<0.05),且Ⅱ组(29.26±5.91)%>Ⅰ组(15.37±1.49)%>Ⅲ组(2.05±0.85)%;3组的血小板CD62P平均荧光强度(MFI)存在差异(P<0.05),且Ⅱ组(3.97±0.64)>Ⅰ组(2.51±0.53)>Ⅲ组(1.50±0.10)。Ⅱ组血小板脱颗粒比Ⅰ组更明显,Ⅲ组未发生脱颗粒。结论 HDL能显著抑制oxLDL体外诱导的血小板活化。Objective To investigate the effect o5 high-density lipoprotein (HDL) on platelet activation induced by oxidized low-density lipoprotein (ox-LDL) in vitro. Methods Washed platelets separated from the patients with coronary heart disease were divided into three groups,the group Ⅰ (adding HDL 100μg/mL) ,the group Ⅱ and group Ⅲ (adding PBS). The platelets of three groups were incubated at 37 ℃ for 15 min. Then,the group Ⅰ and Ⅱ were added with ox-LDL 50 μg/mL and the group Ⅲ was added with PBS. The three groups were incubated at 37℃ for 15 min again. The expression levels of CD62P were assessed by flow Cytometry and compared among three groups. The structures of platelets were observed by transmission electron microscope (TEM) and compared among three groups. Results The CD62P expression rates were statistically different among three groups (P〈0.05) and the sequence was the group Ⅱ (29.26-4-5.91)%〉 the group Ⅰ (15.37±1.49)%〉 the group Ⅲ (2. 05± 0.85)% (P〈0.05). The mean fluorescence intensities (MFI) of CD62P were also statistically different among three groups (P〈 0.05) and the sequence was the group Ⅱ (3.97±0.64)〉 the group Ⅰ (2. 51±0.53)〉 the group Ⅲ (1.50±0.10) (P〈0.05). The degranulation degree in the group Ⅱ was significantly more than that in the group Ⅰ (P〈0.05) ,whereas the group Ⅲ had no degranulation. Conclusion HDL inhibits can significantly the platelet activation induced by ox-LDL in vitro.

关 键 词:冠心病 脂蛋白类 HDL 血小板 氧化低密度脂蛋白 动脉粥样硬化 

分 类 号:R541.4[医药卫生—心血管疾病]

 

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