外源一氧化氮对微囊藻毒素诱导青菜氧化胁迫的缓解  

Alleviation of microcystin-induced oxidative stress by exogenous nitric oxide in Brassica rapa

作　　者：尹干[1] 李慧明[2] 陈健[3,4,5] 薛延丰[3,4,5] 石志琦[1,3,4,5] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]南京大学环境学院,江苏南京210023 [3]江苏省农业科学院食品质量安全与检测研究所,江苏南京210014 [4]江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地,江苏南京210014 [5]农业部农产品质量安全控制技术与标准重点实验室,江苏南京210014

出　　处：《江苏农业学报》2015年第2期253-259,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(21207054);江苏省农业科技自主创新基金项目[CX(13)5052]

摘　　要：为了探讨一氧化氮(NO)参与调控微囊藻毒素(MC)诱导植物损伤的机理,以青菜(Brassica rapa)幼苗为材料,研究了外源NO对MC胁迫下青菜幼苗的生长、体内活性氧(ROS)累积、抗氧化酶活性及抗氧化物质含量的影响及可能的调控机制。结果显示:(1)外源NO供体硝普钠(SNP)预处理能够显著缓解MC对青菜幼苗生长的抑制效应;(2)SNP预处理能够显著降低MC诱导青菜幼苗ROS的过量产生;(3)SNP预处理能够分类调控MC对抗氧化酶(SOD、POD、APX、CAT)和抗氧化物质(As A和GSH)的诱导效应。这些结果表明,外源NO能够通过特异性调控MC诱导青菜幼苗的氧化胁迫反应,进而缓解MC对植物的毒害效应。To study the mechanism underlying nitric oxide （NO） involvement in the regulation of microcystin （MC） induced plant damage, the effects of exogenous NO on the growth of Brassica rapa seedlings, exposed to MC accumulation of reactive oxygen species （ ROS）, activity of several important anti-oxidative enzymes and content of antioxidant substances were measured under MC stress. The pre-treatment with NO donor SNP could significantly attenuate MC-induced growth stunt in B. rapa seedlings and reduce over-accumulation of reactive oxygen species （ROS）. Pre-treatment with SNP could differentially reguate the increases in the activities of anti-oxidative enzvmes （SOD. POD. APX and CAT）. and the content of antioxidants （AsA and GSH） in B. rapa. In conclusion, exogenous NO could alleviate MC-induced injury by classified regulation of oxidative stress in B. rapa.

关 键 词：青菜 微囊藻毒素 氧化胁迫 一氧化氮 

分 类 号：S634.3[农业科学—蔬菜学]