HPLC法测定复方甲氧沙林乳膏中甲氧沙林和醋酸氯已定的含量  

Determination of Content of Methoxsalen and Chlorhexidine Acetate in Compound Methoxsalen Cream by HPLC

作　　者：崔俊凤 郭宇鹏 

机构地区：[1]山东省滨州市药品检验所,山东滨州256618

出　　处：《解放军药学学报》2014年第6期538-540,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立HPLC法同时测定复方甲氧沙林乳膏中甲氧沙林和醋酸氯已定的含量。方法色谱柱Thermo,ODS-2HYPERSIL（250 mm×4.6 mm）;流动相：甲醇-水（70∶30）;流速：1.0 ml·min-1;检测器：DAD检测器;检测波长：249 nm和259 nm;柱温：30℃。结果醋酸氯已定和甲氧沙林线性范围分别为0.1032~2.064μg（r=0.9998）和0.0993~1.986μg（r=0.9999）。平均加样回收率分别为97.7%（RSD=1.2%）和94.4%（RSD=1.6%）。结论本法操作简便、准确、重复性好,回收率高,可用于复方甲氧沙林乳膏的质量控制。Objective To establish an HPLC method for determination of methoxsalen and chlorhexidine acetate in compound methoxsalen cream.Methods The separation was performed on a Thermo,ODS-2HYPERSIL（250 mm × 4.6 mm） column with a moble phase of methanol-water（70∶ 30） at a flow rate of 1.0 ml·min-1.The DAD detection wavelength was 249 nm and 259 nm respectively.The column temperature was 30 ℃.Results The calibration curves of chlorhexidine acetate and methoxsalen were linear in the range of 0.1032-2.064 μg（r = 0.9998）and 0.0993-1.986 μg（r = 0.9999） respectively.The average recovery of chlorhexidine acetate and methoxsalen was 97.7%（RSD = 1.2%.） and 94.4%（RSD = 1.6%） respectively.Conclusion This method is simple,accurate and repeatable,which can be used for quality control of compound methoxsalen cream.

关 键 词：复方甲氧沙林乳膏 醋酸氯已定 甲氧沙林 HPLC 

分 类 号：R927.7[医药卫生—药学]