HPLC法测定天冬降糖胶囊中芒果苷的含量  

Determination of Mangiferin in Tiandong Jiangtang Capsules by HPLC

作　　者：胡丹[1,2] 曹红[2] 邢俊波[2] 陈玉敏[2] 水彩红[2] 单婷婷[2] 姜春来[2] 岳来富 

机构地区：[1]第二军医大学药学院,上海200433 [2]总后勤部卫生部药品仪器检验所,北京2100166 [3]蚌埠医学院,安徽蚌埠233000

出　　处：《解放军药学学报》2014年第6期544-545,共2页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家重大科学仪器设备开发专项项目;No.2012YQ18013208

摘　　要：目的建立天冬降糖胶囊中芒果苷的含量测定方法。方法采用HPLC法,色谱柱为Waters 5C18-MS-Ⅱ（4.6 mm×250 mm,5μm）;流动相为乙腈-0.2%冰醋酸（12∶88）为流动相;流速为1.0 ml·min-1,检测波长258 nm。结果芒果苷含量在0.06~0.48μg范围内线性关系良好（r=0.9999）,平均回收率为97.51%,RSD为1.09%（n=6）。结论该方法简便、准确,重复性好,可用于测定天冬降糖胶囊中芒果苷的含量。Objective To establish a method for determination of mangiferin in Tiandong Jiangtang capsules by HPLC.Methods An external standard method by HPLC with Waters 5C18-MS-Ⅱ（4.6 mm × 250 mm,5μm） column was adopted.The mobile phase was acetonitrile-0.2% acetic acid（12 ∶ 88） at a flow rate of 1.0ml·min-1and a UV detection wavelength of 258 nm.Results The linear range was 0.06-0.48 μg（r = 0.9999）.The average recovery was 97.51% and RSD was 1.09%（n = 6）.Conclusion This method is simple and accurate,which can be employed for quantitative analysis of mangiferin in Tiandong Jiangtang capsules.

关 键 词：天冬降糖胶囊 芒果苷 HPLC 

分 类 号：R927.2[医药卫生—药学]