黄连水提物抗甲型流感病毒活性初步研究  

Effect of Coptis chinensis aqueous extract on activity of influenza A virus

作　　者：马卫列[1] 罗芬[1] 李锦云 陈潇楷 林东子 丁航[1] 张志珍[1] 

机构地区：[1]广东医学院生物化学与分子生物学教研室,广东东莞523808 [2]海南通用三洋药业有限公司,海南海口570312 [3]广东省东莞市慢性病防治院检验科,广东东莞523008

出　　处：《广东医学院学报》2014年第6期759-762,共4页Journal of Guangdong Medical College

基　　金：广东省自然科学基金项目(No.S2011010002984);广东省高等学校人才引进专项资金项目(粤财教【2013】246号);东莞市高等院校科研机构科技计划项目经费资助(No.2011108101007)

摘　　要：目的观察黄连水提物对甲型流感病毒（IAV）活性的影响。方法 CCK-8法检测黄连水提物对狗肾传代（MDCK）细胞的毒性作用。采用反向遗传8质粒病毒拯救系统制备IAV后,CCK-8和CPE法检测黄连水提物抗IAV活性。利用荧光素酶编码基因报告系统研究黄连水提物对IAV感染性和RNA聚合酶活性的影响。结果黄连水提物在0.25~2.0g/L质量浓度内,对MDCK细胞有一定安全性。黄连水提物有较好的抗IAV作用。与对照组比较,黄连水提物处理组细胞的相对荧光强度明显降低（P〈0.01）。结论黄连水提物对IAV活性及感染性有较强抑制作用,可能与抑制病毒RNA聚合酶活性有关。Objective To study the effect of Coptis chinensis aqueous extract（CCAE） on the activity of influenza A virus（IAV）. Methods The cytotoxicity of CCAE on Madin darby canine kidney（MDCK） cells was detected by CCK-8 assay. Antiviral activity of CCAE was determined by CCK-8 and cytopathic effect methods after recombinant IAV plasmid was constructed using reverse genetic 8-plasmid system. Effects of CCAE on viral infectivity and RNA polymerase were analyzed by luciferase reporter system. Results CCAE was safe for MDCK cells at concentrations of 0.25~2.0 g/L. CCAE showed obvious anti- IAV activity. Relative fluorescence intensity of CCAE-treated cells was lower than that of controls（P〈0.01）. ConclusionCCAE can suppress the IAV activity and infectivity, which might be related to RNA polymerase inhibition.

关 键 词：黄连 甲型流感病毒 抗病毒活性 

分 类 号：R282.71[医药卫生—中药学] R373.13[医药卫生—中医学]