柳珊瑚共附生真菌Aspergillus sclertoiorum和Penicillium citrinum共培养代谢产物的研究  

Secondary Metabolites from the Co-culture of Gorgonian-associated Fungi Aspergillus sclertoiorum and Penicillium citrinum

作　　者：鲍洁[1,2] 张晓勇[1] 姚期凤 徐新亚[1] 农旭华[1] 漆淑华[1] 

机构地区：[1]中国科学院南海海洋研究所,中国科学院热带海洋生物资源与生态重点实验室广东省海洋药物重点实验室,广州510301 [2]中国科学院大学,北京100049

出　　处：《天然产物研究与开发》2014年第A01期1-4,共4页Natural Product Research and Development

基　　金：国家自然科学基金（41376160）,广东省海洋经济创新发展区域示范专项（GD2012-D01-002）

摘　　要：利用柱色谱技术，分离纯化来源于柳珊瑚的两株海洋真菌（Aspergillus sclertoiorum和Penicillium cirrinum）共培养发酵代谢产物，通过NMR等波谱学数据进行结构鉴定，并利用HPLC分析A．sclertoiorum分别共培养和纯培养时主要次生代谢产物产量的差异。从共培养的乙酸乙酯提取物中分离鉴定了4个化合物，分别为青霉酸（1），5t6-dihydropenicillic acid（2），6-dihydro-6-hydroxy penicillic acid（3），2-hydmxy-3，6-dimethylbenzoic acid（4），其中化合物4为首次从海洋来源真菌中得到，并且共培养不同程度地提高了化合物1—4的产量。Four compounds were yielded by a microbial co-culture of the gorgonian-associated fungus Aspergillus sclertoiorum and Penieillium eitrinum,including penicillic acid （ 1 ）, 5,6-dihydropenlcillic acid （2）, 6-dihydro-6-hydroxy penicillic acid（3） and 2-hydrexy-3 , 6-dimethylbenzoic acid （4）. The structures of them were elucidated by the analysis of NMR data and comparison with literatures. HPLC was applied for the analysis of the production of 1-4 and they were enhanced in different level in the co-culture compared with A. sclertolorum cultured individually. Compound 4 was obtained from marine fungus for the first time.

关 键 词：柳珊瑚共附生真菌 ASPERGILLUS sclertoiorum PENICILLIUM citrinum 共培养 代谢产物 

分 类 号：Q939.9[生物学—微生物学]