HPLC法快速测定川芎药材中阿魏酸的含量  

Rapid Determination of Ferulic Acid in Ligusticum chuanxiong Rhizomes using High-Performance Liquid Chromatography

作　　者：刘金亮[1,3] 郑顺林[1,3] 范巧佳[2] 袁继超[1,3] 杨世民[1,3] 孔凡磊[1,3] 

机构地区：[1]四川农业大学农学院,成都611130 [2]四川农业大学动物医学院,雅安625014 [3]农业部西南作物生理生态与耕作重点实验室,成都611130

出　　处：《天然产物研究与开发》2014年第A01期67-71,共5页Natural Product Research and Development

摘　　要：本文建立了快速测定川芎药材中阿魏酸含量的方法。以阿魏酸为标准品。采用Agilent 1100高效液相色谱仪和Waters Syrmnetry C18色谱柱，以乙腈和1％冰乙酸（32：68）为流动相，检测波长为321nm。经测定阿魏酸浓度在0．1—160μg／mL范围内呈良好的线性关系（r=O．9999），平均回收率在96．88—99．75％之间，检测限和定量限分别为0．0043μg／mL和0．0128μmL。该方法简便、准确、重现性好，可作为快速检测川芎药材中阿魏酸含量的方法。We developed a simple and rapid method for determining ferulic acid in Ligusticum chuanxiong rhizomes. Ferulic acid was extracted from the samples using methanol and analyzed using reversed-phase high performance liquid chromatography（HPLC）. The extract was chromatographically separated using an Agilent 1100 series HPLC system with a Waters Symmetry Cls column and isocratically e.luted with a mixture of acetonltrile and 1% aqueous acetic acid（32： 68,v/v）. The effluent was monitored using a VWI） detector set at 321 ran. The average recovery rates ranged farm 96. 88% to 99.75% （ n = 3）. The limit of detection was 0.0043 μg/mL and the limit of quantification was 0. 0128 p,g/mL. The method has been successfully applied to analyze L chuanxiong samples. The ferulic acid content of 38 samples variod from 0.41 mg/g to 3.12 mg/g.

关 键 词：魏酸 HPLC 川芎 含量 方法建立 

分 类 号：R284.2[医药卫生—中药学]