从喜树叶中连续分离喜树碱和羟基喜树碱  

A Method of Separating Camptothecin and 10-Hydroxycamptothecin from Leaves of Camptotheca Acuminate Decne in a Row

作　　者：罗虹[1] 戴汉松[1] 王家文[2] 王涛[3] 

机构地区：[1]四川师范大学化学与材料科学学院,成都610068 [2]四川大学华西医院国家重点实验室,成都610041 [3]四川理工学院化学系,自贡643000

出　　处：《天然产物研究与开发》2014年第A02期231-233,共3页Natural Product Research and Development

摘　　要：以喜树叶为原料，用0．3％NaOH碱法提取，加入生石灰过滤去除果胶，用乙醇萃取滤液，然后用盐酸调pH=3。经柱层析用梯度淋洗法可分别得到喜树碱和10-羟基喜树碱，淋洗条件为：2％甲醇的氯仿溶液和4％甲醇的氯仿溶液。喜树碱的收率为O．34‰，羟基喜树碱的收率为0．02‰。喜树碱和羟基喜树碱的纯度均在98．5％以上。The leaves of camptotheea acuminate decne are used as the raw material for extraction of eamptothecin and 10- hydroxycamptothecin. The leaves are dipped in 0. 3% NaOH. Quicklime is added to precipitate the pectin and filtrate it. The liquid is extracted by ethyl alcohol. Then the filtrate liquid is become pH = 3 though dropping in hydrochloric acid. The camptothecin and 10-hydroxycamptothecin can be gotten by the 2% and 4% methanol in the chloroform through the silica chromatogram. The rate of yield of camptothecin and 10-hydroxycamptothecin is 0.34‰ and 0.02‰ respectively, and their purity all exceed 98 % （HPLC）.

关 键 词：喜树叶 喜树碱 10-羟基喜树碱 分离 

分 类 号：O629.9[理学—有机化学]