红花花冠总RNA提取方法的筛选研究  

The Screening Study of Method of Total RNA Extraction of Safflower Corolla

作　　者：康亚兰[1,2] 裴瑾[1] 刘薇[1] 罗静[1] 刘维[1] 陈翠平[1] 吴清华[1] 

机构地区：[1]成都中医药大学药学院,中药资源系统研究与开发利用国家重点实验室培育基地,成都610075 [2]成都医学院药学院,成都610083

出　　处：《天然产物研究与开发》2014年第A02期313-316,331,共5页Natural Product Research and Development

基　　金：四川省教育厅重点项目（14ZA0074）

摘　　要：筛选一种红花花冠总RNA提取方法。采用植物总RNA提取试剂盒法、传统佰烈法、改良Trizol法提取红花花冠总RNA。凝胶电泳、RT-PCR、紫外分光光度法检测三种方法提取所得总RNA，并将筛选得到的方法应用于红花愈伤组织总RNA的提取。改良Trizol法提取得到的红花花冠总RNA凝胶电泳检测可见清晰的三条带，RT-PCR结果凝胶电泳检测可见清晰的一条带，紫外分光光度法检测符合要求，其余两种方法均不符合要求。改良Trizol法应用于红花愈伤组织总RNA提取，检测结果均符合要求。改良Trizol法可用于红花花冠总RNA提取，提取所得红花花冠总RNA可用于后续分子生物学实验。A method used for the extraction of safflower corolla was screened. Plant total RNA extraction kit,traditional Trizol ,improved Trizol were used for the extraction of safflower coroUa. Gel electrophoresis, RT-PCR and ultraviolet spectroscopy were used for detection. And the method screened was used for the total RNA extraction of safflower callus. The total RNA extraction of safflower Corolla used improved Trizol obtained clearly visible gel electrophoresis three bands, RT-PCR results visible gel electrophoresis with a clear, UV spectrophotometry meet the requirements, the other two methods do not meet the requirements. The improved Trizol can used for the extraction of saffiower corolla,the obtained total RNA can be used for the following experiment of Molecular Biology.

关 键 词：红花花冠 总RNA提取 植物总RNA提取试剂盒 传统Trizol法 改良Trizol法 

分 类 号：R284.2[医药卫生—中药学]