利用“微创刷”法获得抗草甘膦转基因大豆  

Research of “Minimal Wound Brush”to Acquire Genetically Modified Glyphosate- resistant Soybean

作　　者：荣非 王罡[1] 季静[1] 王萍[2] 董歆[2] 高海伶[1] 曹越平[3] 邱丽娟[4] 

机构地区：[1]天津大学环境科学与工程学院,天津300072 [2]淮海工学院海洋学院,江苏连云港222005 [3]上海交通大学农业与生物学院.上海200436 [4]中国农业科学院作物科学研究所,北京100081

出　　处：《大豆科学》2015年第2期217-223,共7页Soybean Science

基　　金：国家转基因生物新品种培育重大专项(2011ZX08004-001-04)

摘　　要：以发芽3 d的大豆成熟种子胚尖生长点为作用点,利用"微创刷"法将抗草甘膦基因(EPSPS)转入绥农22中,对转化植株T1代进行草甘膦筛选,对筛选后的抗性植株进行PCR检测,得到抗草甘膦转基因大豆。同时研究了不同浓度草甘膦对野生型绥农22与抗草甘膦转基因绥农22大豆植株的影响。结果表明:绥农22 T0代成株率为97.38%,对T1代具有草甘膦抗性的植株进行PCR检测,初步证明EPSPS基因成功转入大豆中,T1代转化效率为6.20%;对野生型绥农22与"微创刷"法获得的转基因绥农22大豆在不同浓度草甘膦进行相关生理指标测定,抗草甘膦转基因绥农22大豆在不同浓度草甘膦作用下叶片叶绿素含量指数、光合速率高于野生型绥农22大豆,莽草酸含量低于野生型绥农22大豆,进一步证明了大豆抗性植株对草甘膦的抗性。Growing points of embryonic tips from mature soybeans after 3 days post germination were used as a receiving point of Agrobacteria carrying recombinated vectors for the transformation of glyphosate - resistant gene （EPSPS） into soybeans （ Suinong 22） by using ＂minimal wound brush＂ method, and transformed T1 plants were screened by glyphosate and tested by PCR. Chlorophyll content index, shJkimJc acid concentration, and photosynthetic rate of wild -type and transgenic soybean plants were determined. The results showed that surviving rate of To soybean plants was 97. 38%, transformation rate for T~ soybean plants was 6. 20%. Meansurements of related physiological factors from wild - type and transgenic plants were taken and it was found that chlorophyll content index and photosynthetic rate were higher in trangenic plants than wild types; mean- while, shikimic acid concentration were much lower in transgenic plants than wild types. The above physicological results con- firmed the resistance of glvohosate in transgenic sovbeans plants.

关 键 词：大豆 胚尖生长点 “微创刷” EPSPS基因 

分 类 号：S565.1[农业科学—作物学]