3种新型氧钒配合物的抗肿瘤活性作用评价  

Evaluation of three oxovanadium complexes on antitumor activities in vitro

作　　者：田旭岩[1] 应鹏[2] 陆家政[2] 陈宏远[1] 芮雯[3] 

机构地区：[1]广东药学院基础学院,广东广州510006 [2]广东药学院药科学院,广东广州510006 [3]广东药学院中心实验室,广东广州510006

出　　处：《广东药学院学报》2015年第2期247-252,共6页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的评价自行设计合成的3种氧钒配合物体外抗肿瘤活性作用。方法采用MTT法研究3种氧钒配合物：[VO（hntdtsc）（CF3PIP）]（1）、[VO（hntdtsc）（m-CF3PIP）]（2）和[VO（hntdtsc）（pCF3PIP）]（3）对人宫颈癌、食管癌和乳腺癌细胞系在体外生长的抑制作用。采用流式细胞术检测其对食管癌细胞系的凋亡诱导作用和细胞周期阻滞作用。结果 3种新合成的化合物与肿瘤细胞系作用时细胞毒性的IC50值在0.31~6.15μmol/L,接近于顺铂（0.52~2.49μmol/L）。化合物2和3能抑制食管癌细胞系的细胞G0/G1期和S期阻滞并诱导其发生凋亡。结论含氟缩氧硫脲类希夫碱氧钒配合物具有良好的抗肿瘤活性,其机制可能与其诱导细胞凋亡相关。Objective To evaluate three novel synthesized oxovanadium complexes＇ antitumor activities in vitro. Methods MTI＇ methods was used to detect the inhibition of three oxovanadium complexes, including [ VO（ hntdtsc ） （ CF3PIP ） ], [ VO （ hntdtsc ） （ m-CF3PIP ） ], and [ VO （ hntdtsc ） （p-CF3PIP） ] on human cervical cancer, esophagus cancer, and breast cancer cell lines. Flow cytometry was used to analyze the cell apoptosis and cell cycle. Results Three complexes exhibited obviously antiproliferative activity against human cervical cancer, esophagus cancer, and breast cancer cell lines.ICso values of three complexes were low （0.31 to 6.15 μmol/L）, which was close to that of cisplatin （0.52-2.49 μmoL/L）. Complexes 2 and 3 induced G0/Gj and S phase arrest and cell apoptosis in esophagus cancer cells. Conclusion Three oxovanadium complexes exhibit considerable inhibitory effects on tumor cells, which mechanism may be related to induction of cell apoptosis.

关 键 词：氧钒配合物 凋亡 抗肿瘤 

分 类 号：R96[医药卫生—药理学]