RP-HPLC测定苎麻不同部位中3个三萜酸异构体的含量  

Determination of Three Isomers of Triterpenic Acid in Different Parts of Boehmeria nivea by RP- HPLC

作　　者：邹盛勤[1] 周兰姜[1] 

机构地区：[1]江西省天然药物活性成分研究重点实验室宜春学院化学与生物工程学院,江西宜春336000

出　　处：《宜春学院学报》2015年第3期4-6,共3页Journal of Yichun University

基　　金：国家高技术研究发展计划资助项目(2002AA2Z3217);江西省科技攻关计划项目(2007BG07200)

摘　　要：目的:建立了RP-HPLC法同时测定苎麻不同部位中3个同分异构体白桦脂酸、齐墩果酸和熊果酸的含量。方法:色谱柱为Kromasil C18柱(4.6mm×250mm,5μm),以乙腈∶水∶乙酸(80∶20∶0.2)为流动相,流速1.0m L/min;检测波长210 nm;进样量20μL;柱温25℃。结果:白桦脂酸、齐墩果酸和熊果酸在20min内均达到基线分离,各成分在其线性范围内线性关系良好,线性范围分别为3.83-229.80μg/m L(r1=0.9999)、0.92-55.20μg/m L(r2=0.9998)和2.35-141.00μg/m L(r3=0.9999),平均回收率(n=6)分别为100.4%(RSD=1.9%)、98.0%(RSD=2.2%)和96.2%(RSD=1.6%)。结论:方法准确,操作简便,可将苎麻中3个三萜酸异构体一次性分离定量。Objective： To establish a convenient method for simultaneous determination of betulinic acid, oleanol- ic acid and ursolic acid in different parts ofBoehmeria nivea. Methods： The separation was achieved on a Kroma- sil Cls column （4. 6mm x250mm, 5p^n） using acetonitrile -water -acetic acid solution （80： 20： 0. 2） as the mobile phase at a flow rate of 1.0mL/min. The detection wavelength and column temperature were set at 210 nm and 25~C, respective, and the sample size was 20p＆. Results： Betulinic acid, oleanolic acid and ursolic acid were simultaneously achieved within 20 min under the above conditions. A good relationship was observed in the range of 3.83 -229.80txg/mL （rl =0. 9999）, 0. 92 -55.20~g/mL （r2 =0. 9998） and 2.35 - 141. 001~g/mL （r3 =0. 9999） for betulinic acid, oleanolic acid and ursolic acid, respectively, with the average recoveries of 100. 4 % （ RSD = 1.9% ）, 98.0% （ RSD =2. 2% ） and 96. 2% （ RSD = 1.6% ） . The proposed method is ac- curate and simple. Conclusions： Three isomers of triterpenic acid are separated and quantitative determined simul- taneously, accurately and effectively in Boehrneria nivea.

关 键 词：苎麻 三萜酸 白桦脂酸 齐墩果酸 熊果酸 RP-HPLC 

分 类 号：R284[医药卫生—中药学]