FAVN与RFFIT在动物和人狂犬病中和抗体检测中的比较  

Comparison between FAVN and RFFIT for rabies neutralizing antibody detection of sera from animals and human

作　　者：张守峰[1] 张菲[1] 刘晔[1] 王颖[1] 米立娟[1] 扈荣良[1] 

机构地区：[1]军事医学科学院军事兽医研究所吉林省人兽共患病防控重点实验室,吉林长春130122

出　　处：《国际检验医学杂志》2015年第9期1161-1163,共3页International Journal of Laboratory Medicine

基　　金：狂犬病等重大动物源性嗜神经病毒新型疫苗创制项目(863项目)(2012AA101303)

摘　　要：目的比较荧光抗体病毒中和试验(FAVN)和快速荧光灶抑制试验(RFFIT)两项技术用于动物和人血清狂犬病中和抗体检测的差异。方法以FAVN和RFFIT两种方法分别对60份动物及人血清进行狂犬病中和抗体的平行定量检测,应用配对定量资料的t检验对所得数据进行统计学分析。结果以0.5IU/mL的国际推荐标准,对同一样品经FAVN和RFFIT两种方法测定的中和抗体效价进行定性判定,二者对全部60份样品的检测符合率为96.67%(58/60)。统计学分析显示,两种检测方法对总体样品及不同动物样品的定量检测结果差异均无统计学意义(P>0.05)。结论 FAVN和RFFIT可通用于动物和人血清的狂犬病中和抗体检测。Objective To compare the differences of fluorescent antibody virus neutralization test （FAVN） and rapid fluores- cence focus inhibition test （RFFIT） in detecting rabies neutralizing antibody of animals and humans. Methods The neutralizing an- tibody titer against rabies virus in 60 animal sera and human sera were performed the parallel and quantitative detection by FAVN and RFFIT respectively. Significance analysis between the paired quantitative data was performed by t test. Results The coinci- dence rate between the qualitative results of FAVN and RFFIT was 96.67% （58/60） at the cutoff value of 0.5 IU/mL. The quantitative detection results of the overall samples and different animal samples had no statistically significant difference between FAVN and RFFIT （P〈0.05）. Conclusion , FAVN and RFFIT could be commonly used in the rabies neutralizing antibody titer detection of animal sera and human sera.

关 键 词：狂犬病 中和抗体 荧光抗体病毒中和试验 快速荧光灶抵制试验 比较 

分 类 号：R512.99[医药卫生—内科学] R446.6[医药卫生—临床医学]