3种基因分型方法在鲍曼不动杆菌中的应用评价  

Application evaluation of three kinds of genotyping methods in Acinetobacter baumannii

作　　者：姜飞[1] 邓丽华[1] 康海泉[1] 赵晓杰[1] 马萍[1] 李洪春[1] 

机构地区：[1]徐州医学院附属医院检验科,江苏徐州221002

出　　处：《国际检验医学杂志》2015年第9期1195-1197,共3页International Journal of Laboratory Medicine

基　　金：江苏省徐州市科技局资助项目(XZZD1326)

摘　　要：目的比较3种基因分型方法在鲍曼不动杆菌中的应用。方法采用琼脂稀释法检测重症监护室（ICU）分离的30株鲍曼不动杆菌的最小抑菌浓度（MIC）;分别以肠杆菌科基因间重复一致性序列（ERIC）、基因外回文重复序列（REP）及随机多态性核苷酸序列（RAPD）为引物进行PCR扩增,利用电泳指纹图谱进行基因分型,并进行聚类分析。结果 30株鲍曼不动杆菌仅对头孢哌酮/舒巴坦有较低的耐药率;ERIC-PCR基因分型法的扩增条带最为丰富,能扩增出4∽7个条带,RAPD和REP-PCR扩增条带较少,分别扩增出1∽5和1∽4个条带;3种方法分别将30株鲍曼不动杆菌分为4、4、3个群。结论头孢哌酮/舒巴坦对于多重耐药鲍曼不动杆菌依然有较好的敏感性;本试验初步证实3种方法均可作为鲍曼不动杆菌基因分型的可选方法,ERIC-PCR较另外两种方法扩增条带更丰富,分辨率更好。Objective To compare the application of three kinds of genotyping methods in Acinetobacter baumannii. Methods The minimum inhibitory concentration （MIC） of 30 strains of Acinetohacter baumannii collected from ICU was detected by adop- ting the agar dilution method. Thirty Acinetobacter baumannii strains were genotyped respectively by enterobaeterial repetitive in tergenie consensus sequences （ERIC）, repetitive extragenie palindromic（REP） and nueleotide random amplified polymorphie se quence （RAPD） as the primers for PCR amplification. Genotyping was performed using electrophoresis fingerprint,and clustering analysis. Results Thirty strains of Acinetobacter haumannii had the low resistance rate only to cefoperazone / sulhaetam, ERIC PCR genotyping method could amplify out 4--7 bands,the most abundant amplification bands. Bands amplified by RAPD and REP PCR were less, 1- 5 and 1- 4 bands were amplified out respectively. Thirty strains of Acinetobaeter baumannii could be divided in- to 4,4 and 3 groups by three methods respectively. Conclusion Cefoperazone / sulbactam are still better sensitivity to multiple drug resistant Acinetobacter baumannii. The test preliminary confirms that all of the three kinds of methods can be used as alternative method for genotyping Acinetobaeter baumanniL Compared with the other two methods, bands of ERIC PCR is more abundant and the resolution is better.

关 键 词：肠杆菌科基因间重复一致性序列 随机多态性核苷酸序列 基因外回文重复序列 鲍曼不动杆菌 基因分型 

分 类 号：R446.5[医药卫生—诊断学]