乙型肝炎肝硬化患者外周血MIF、IL-17和IL-10检测的临床意义  

Clinical significance of detecting peripheral blood MIF,IL-17 and IL-10 in patients with liver cirrhosis

作　　者：曾钢[1] 李彩东[1] 吴斌[1] 杨勇卫[1] 

机构地区：[1]甘肃省兰州市第二人民医院,甘肃兰州730046

出　　处：《国际检验医学杂志》2015年第9期1205-1206,1209,共3页International Journal of Laboratory Medicine

基　　金：兰州市科技发展指导性计划项目(2013-ZD-07)

摘　　要：目的探讨巨噬细胞移动抑制因子(MIF)、白介素-17(IL-17)和白介素-10(IL-10)在乙型肝炎肝硬化发生、发展中的作用机制。方法采集60例乙型肝炎肝硬化患者和50例健康对照者空腹血清标本,采用ELISA法检测血清细胞因子MIF、IL-17和IL-10水平;采用荧光定量PCR法测定各组HBV DNA载量;采用全自动生化分析仪检测血清肝功能水平;进行临床相关性分析。结果与对照组相比,乙型肝炎肝硬化患者ALT、AST、胆红素、GLB水平明显升高,差异有统计学意义(P<0.05);与对照组相比,乙型肝炎肝硬化患者MIF、IL-17和IL-10水平明显升高,差异有统计学意义(P<0.05);IL-17水平与TBIL、DBIL、IBIL、TP和GLB水平呈正相关,IL-10水平与TBIL、DBIL、IBIL、TP和GLB水平亦呈正相关。结论 MIF、IL-17和IL-10间接或直接的参与了肝硬化的发生发展。Objective To investigate the action mechanisms of macrophage migration inhibitory factor（M1F） ,interleukin-17（IL- 17） and interleukin-10（IL-10） in the occurrence and development of liver cirrhosis. Methods The fasting serum samples were col- lected from 60 cases of liver cirrhosis and 50 healthy controls. ELISA was used for detecting serum cytokine MIF,IL-17 and IL-10 levels;The HBV DNA load was measured by fluorescence quantitative PCR〈the liver function was tested by the automatic biochemical analyzer. Then the clinical correlation analysis was performed. Results Compared with the healthy control group, the ALT, AST,bilirubin and GLB levels in liver cirrhosis group were significantly increased with statistical differences between the two groups（P〈0.05）. Compared with the healthy control group, the MIF,IL-17 and IL-10 levels in the liver cirrhosis group were sig- nificantly increased with statistical differences between them（P〈0.05）. There were positive correlation between the IL-17 level with the TBIL,DBIL, IBIL ,TP and GLB levels and between the IL-10 level with the TBIL, DBIL, 1BIL, TP and GLB levels. Conclu- sion MIF,IL-17 and IL-10 directly or indirectly participate in the occurrence and development of liver cirrhosis.

关 键 词：乙型肝炎 肝硬化 巨噬细胞移动抑制因子 白介素-17 白介素-10 

分 类 号：R512.62[医药卫生—内科学] R446.1[医药卫生—临床医学] R440