甘青乌头抗炎镇痛活性部位的半仿生-酶法提取工艺优化  

Optimization of extracting technology semi bionic-enzyme for anti-inflammatory and analgesic activity components in Aconitum tanguticum

作　　者：吴国泰[1,2] 王瑞琼[2,3] 邵晶[2] 杜丽东[2] 尚若峰[1] 景琪[2] 梁剑平[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730000 [2]甘肃中医学院甘肃省中药药理与毒理学重点实验室,甘肃兰州730000 [3]甘肃农业大学,甘肃兰州730070

出　　处：《应用化工》2015年第4期605-610,共6页Applied Chemical Industry

基　　金：国家"十一五"科技支撑计划项目(2011AA10A214);甘肃省中药药理学和毒理学重点实验室开放基金项目(ZDSYS-KJ-2012-004)

摘　　要：用半仿生-酶法提取甘青乌头抗炎镇痛的活性部位,考察加酶量、酶解时间、半仿生提取(SBE)温度和总时间4个因素的影响。结果表明,优选工艺为:加酶量36 U/g,酶解时间90 min,SBE温度80℃,SBE总时间180 min。Semi-bionic to extract activity component from Aconitum tanguticum.The influence of enzyme dosage ,enzymatic time ,SBE temperature and total time were investigated .The result showed that the opti-mum process were as follows：36 U/g mixed enzyme ,hydrolysis 90 min then stirring extracted for 180 min under 80 ℃.

关 键 词：甘青乌头 抗炎镇痛 响应面法 半仿生-酶法 提取工艺 

分 类 号：TQ016.1[化学工程]