药用菊花叶片主要活性成分比较  

Comparison of major bioactive components from leaves of Chrysanthemum morifolium

作　　者：汪涛[1,2] 沈学根 郭巧生[1] 周建松 毛鹏飞[1] 沈振国[2] 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095 [2]南京农业大学生命科学学院生物学博士后流动站,江苏南京210095 [3]桐乡农业推广中心,浙江嘉兴314500

出　　处：《中国中药杂志》2015年第9期1670-1675,共6页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09308-002)

摘　　要：对不同栽培类型药用菊花叶片主要活性成分进行分析,为进一步中药新资源开发利用提供依据。该研究以UV-VIS测定不同栽培类型药用菊花叶片总黄酮(TF)含量,HPLC测定木犀草苷(GA),槲皮苷(QU),绿原酸(CA),3,5-O-二咖啡酰奎宁酸(CQ)的含量。结果显示安徽歙县晚贡菊TF量最高为7.13%;安徽亳州大亳菊GA量最高为33.45 mg·g-1;江苏射阳红心菊QU量最高为29.25 mg·g-1;江苏射阳长瓣菊CA量最高为13.14 mg·g-1;浙江桐乡大洋菊CQ质量分数最高为7.35mg·g-1。不同产地不同栽培类型药用菊花叶片中主要活性成分含量差异显著,且不同产地相同栽培类型之间差异亦显著,结果表明药用菊花叶片可以作为中药新资源开发的新来源。Leaves of Chrysanthemum morifolium were potential medicinal resource. The present study aims to estimate the main bio- active components： total flavonoids （TF）, galuteolin （GA）, quercitrin （QU）, chlorogenic acid （CA） and 3,5-O-caffeoylquinic acid （CQ） , which were considered to be the main effective components, in leaves of C. morifolium cultivars in China. The TF content was estimated by UV-VIS spectrophotometry, while GA, QU, CA, and CQ were quantitatively determined by HPLC. The highest TF con- tent （7. 13% w/w） was found in cultivar Wan Gong （Shexian county）. Cuhivar Da Bo （Bozhou county） had the highest GA content （33. 45 mg·g^-1 ） ; Cuhivar Hong Xin （Sheyang county） contained the highest Qu content （29. 25 mg·g^-1 ） ; Cultivar Chang Ban （Sheyang county） had the highest CA content （13.14 mg·g^-1 ）. The maximum CQ content （7.35 mg·g^-1） was observed in culti- var Da Yang （Tongxiang county）. Different cultivars of C. morifolium had significant difference in components, but the leaf and capit- ulum of C. morifolium, were found to possess similar chemical compositions. The high content of bioactive components in several culti- vars suggested the potential utilization of C. morifolium leaves.

关 键 词：药用菊花叶片 黄酮 咖啡酸衍生物 木犀草苷 

分 类 号：S567.239[农业科学—中草药栽培]