绞股蓝属植物亲缘关系初步分析  

Analysis phylogenetic relationship of Gynostemma(Cucurbitaceae)

作　　者：秦双双[1] 李海涛 汪周勇[3] 崔占虎[4] 余丽莹[1] 

机构地区：[1]广西壮族自治区药用植物园,广西南宁530023 [2]中国医学科学院北京协和医科大学药用植物研究所云南分所,云南景洪666100 [3]中国中医科学院中药资源中心,道地药材国家重点实验室培育基地,北京100700 [4]南阳理工学院,河南南阳473004

出　　处：《中国中药杂志》2015年第9期1681-1687,共7页China Journal of Chinese Materia Medica

基　　金：环保部生物多样性保护专项(2013);中医药部门公共卫生专项(GXZYZYPC-1);广西科学研究与技术开发计划课题(桂科攻111107-10-3-3)

摘　　要：采用DNA条形码ITS,mat K,rbc L,psb A-trn H 4个片段,对绞股蓝属Gynostemma 9个种及变种共38份样品的序列进行分析,以雪胆属罗锅底Hemsleya macrosperma作为外类群,运用PAUP 4.0b10软件进行系统发育分析,基于ITS序列采用邻接法(NJ)和最大简约法(MP)构建系统发育树,探讨绞股蓝属下的亲缘关系。研究表明:1在NJ树和严格一致树中,广布种绞股蓝G.pentaphyllum var.pentaphyllum的8个个体没有形成单系分支;2怀疑长梗绞股蓝G.longipes和光叶绞股蓝G.laxum是否应为独立的种;3支持绞股蓝属植物亚属的分类单位。The sequences of ITS, matK, rbcL and psbA-trnH of 9 Gynostemma species or variety including 38 samples were com- pared and analyzed by molecular phylogeny method. Hemsleya macrosperma was designated as outgroup. The MP and NJ phylogenetic tree of Gynostemma was built based on ITS sequence, the results of PAUP phylogenetic analysis showed the following results： ①The eight individuals of G. pentaphyllum var. pentaphyllum were not supported as monophyletie in the strict consensus trees and NJ trees. ②It is suspected whether G. longipes and G. laxum should be classified as the independent species. ③The classification of subgenus units of Gynostemma plants is supported.

关 键 词：绞股蓝 ITS 序列分析 系统发育树 

分 类 号：S567.237[农业科学—中草药栽培]